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  Identification of fibronectin as a major factor in human serum to recruit subchondral mesenchymal progenitor cells

Kulawig, R., Kruger, J. P., Klein, O., Konthur, Z., Schutte, H., Klose, J., et al. (2013). Identification of fibronectin as a major factor in human serum to recruit subchondral mesenchymal progenitor cells. International Journal of Biochemistry and Cell Biology, 45(7), 1410-1418. doi:DOI 10.1016/j.biocel.2013.04.016.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-02DB-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-02DE-6
Genre: Journal Article

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2013
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2013 Elsevier Ltd. All rights reserved.
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 Creators:
Kulawig, R., Author
Kruger, J. P., Author
Klein, O., Author
Konthur, Z.1, Author              
Schutte, H., Author
Klose, J., Author
Kaps, C., Author
Endres, M., Author
Affiliations:
1In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479653              

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Free keywords: cell migration cell recruitment subchondral mesenchymal progenitor cells fibronectin serum fractionation stimulate chemotactic migration therapy position statement stem-cells bone-marrow synovial-fluid follow-up chondrogenic differentiation international-society rheumatoid-arthritis structural proteins
 Abstract: Human serum has the potential for mesenchymal progenitor cell recruitment in repair of articular cartilage lesions. It is unclear which factor(s) in serum mediate this migratory effect. Our goal was to identify cell recruiting factors in human serum fractions obtained by ion exchange chromatography. The recruiting activity of serum fractions on human subchondral mesenchymal progenitor cells was analyzed using 96-well chemotaxis assays. Protein composition of recruiting serum fractions were analyzed by mass spectrometry and showed 58 potential candidates. Fibronectin, gelsolin, lumican, thrombospondin-1 and WNT-9a were identified as key candidates for progenitor cell recruitment. Only human plasma derived and recombinant fibronectin showed significant recruiting activity on progenitors reaching 50-90% of the recruiting activity of normal human serum. Presence of fibronectin in all human serum fractions with recruiting activity was verified by Western blot analysis. This study shows that fibronectin is a key factor in human serum to recruit mesenchymal progenitor cells and might be involved in subchondral mesenchymal progenitor cell migration into cartilage defects after microfracture. (C) 2013 Elsevier Ltd. All rights reserved.

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Language(s): eng - English
 Dates: 2013-04-122013-01-212013-04-142013-04-212013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000321229100028
DOI: DOI 10.1016/j.biocel.2013.04.016
ISSN: 1357-2725
URI: ://WOS:000321229100028http://ac.els-cdn.com/S1357272513001210/1-s2.0-S1357272513001210-main.pdf?_tid=dfbc7fe0-be55-11e3-85ed-00000aacb35e&acdnat=1396876358_8b87f6d38df261483c565881acbeca9a
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Title: International Journal of Biochemistry and Cell Biology
Source Genre: Journal
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Publ. Info: Exeter, England : Pergamon
Pages: 9 Volume / Issue: 45 (7) Sequence Number: - Start / End Page: 1410 - 1418 Identifier: ISSN: 1357-2725
CoNE: https://pure.mpg.de/cone/journals/resource/954927542149