English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  BMP10 as a potent inducer of trophoblast differentiation in human embryonic and induced pluripotent stem cells

Lichtner, B., Knaus, P., Lehrach, H., & Adjaye, J. (2013). BMP10 as a potent inducer of trophoblast differentiation in human embryonic and induced pluripotent stem cells. Biomaterials, 34(38), 9789-9802. doi:DOI 10.1016/j.biomaterials.2013.08.084.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-01FF-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-0201-5
Genre: Journal Article

Files

show Files
hide Files
:
Lichtner.pdf (Publisher version), 6MB
 
File Permalink:
-
Name:
Lichtner.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Molecular Genetics, Berlin; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
2013
Copyright Info:
2013 Elsevier Ltd. All rights reserved.
License:
-

Locators

show

Creators

show
hide
 Creators:
Lichtner, B.1, Author              
Knaus, P., Author
Lehrach, H.2, Author              
Adjaye, J.1, Author              
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Content

show
hide
Free keywords: embryonic stem cells induced pluripotent stem cells bone morphogenetic proteins (bmps) bmp4, bmp5, bmp6, bmp7, bmp10, bmp13 signal transduction trophoblast differentiation bone morphogenetic proteins undifferentiated growth transcription factor signal-transduction expression placenta invasion gene fibroblasts activation
 Abstract: Bone morphogenetic proteins (BMPs) are known to induce diverse differentiation fates in human embryonic stem cells (hESCs). In the present study, we compared the potency at which BMP5, BMP10 and BMP13, which are members of distinct BMP subgroups due to differences in sequential and structural homology, induce differentiation in hESCs and human induced pluripotent stem cells (hiPSCs). We observed, in agreement with previous BMP4 model studies, that all ligands induced differentiation to the trophoblast lineage in the absence of bFGF. However, distinct BMPs exerted differences in the kinetics of induced differentiation, with BMP10 being the most potent. hiPSCs and hESCs shared comparable expression patterns of BMP type-I and -II receptor subtypes, which might explain conserved properties with respect to ligand potency and activation of SMAD-dependent (via SMAD1/5/8) and -independent (via MAPK p38) signal transduction pathways. The tested BMPs had distinct and also conserved target genes such as CDX2, DLX3, DIX5, GATA2, GATA3, HAND1, ID2, MSX2 and TFAP2A, known to be associated with the emergence of trophoblast cells. hESCs induced expression of the BMP antagonist NOGGIN as a protection mechanism to constrict extensive BMP action. Unlike BMP4, BMP10 has been shown to be resistant to NOGGIN-induced inhibition which in part might explain its potency. BMPs, in particular BMP4, are commonly used cytokines in differentiation protocols to generate diverse mesoderm- and endoderm-derivates from human pluripotent stem cells. Our study has identified BMP10, a cardiac-specific protein, as a superior alternative to BMP4 for inducing trophoblast differentiation in human pluripotent stem cells. (C) 2013 Elsevier Ltd. All rights reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2013-07-082013-08-272013-09-232013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: Other: WOS:000328094600007
DOI: DOI 10.1016/j.biomaterials.2013.08.084
ISSN: 0142-9612
URI: ://WOS:000328094600007http://ac.els-cdn.com/S0142961213010624/1-s2.0-S0142961213010624-main.pdf?_tid=b2130176-bb40-11e3-9275-00000aacb35d&acdnat=1396537409_29f0c4586d832345b8fddcfd0c2354f7
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biomaterials
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Guildford, England : Elsevier
Pages: 4 Volume / Issue: 34 (38) Sequence Number: - Start / End Page: 9789 - 9802 Identifier: ISSN: 0142-9612
CoNE: /journals/resource/954925472369