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  From essential to persistent genes: a functional approach to constructing synthetic life

Acevedo-Rocha, C. G., Fang, G., Schmidt, M., Ussery, D. W., & Danchin, A. (2013). From essential to persistent genes: a functional approach to constructing synthetic life. Trends in Genetics, 29(5), 273-279. doi:10.1016/j.tig.2012.11.001.

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 Creators:
Acevedo-Rocha, Carlos G.1, 2, Author              
Fang, Gang3, Author
Schmidt, Markus4, 5, Author
Ussery, David W.6, Author
Danchin, Antoine7, 8, Author
Affiliations:
1Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              
2Univ Marburg, Fachbereich Chemie, D-35043 Marburg, ou_persistent22              
3Yale Univ, New Haven, CT 06520 USA, ou_persistent22              
4Int Dialogue & Conflict Management, A-1070 Vienna, ou_persistent22              
5Biofact KG, A-1160 Vienna, ou_persistent22              
6Tech Univ Denmark, Ctr Biol Sequence Anal, Dept Syst Biol, DK-2800 Lyngby, ou_persistent22              
7Univ Hong Kong, Li KaShing Fac Med, Pokfulam, Hong Kong, Peoples R China, ou_persistent22              
8University of Hong Kong, Li KaShing Faculty of Medicine, 21 Sassoon Road, Pokfulam, Hong Kong, ou_persistent22              

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Free keywords: LUCA; minimal cell; minimal genome; synthetic genomics; chassis; xenobiology
 Abstract: A central undertaking in synthetic biology (SB) is the quest for the 'minimal genome'. However, 'minimal sets' of essential genes are strongly context-dependent and, in all prokaryotic genomes sequenced to date, not a single protein-coding gene is entirely conserved. Furthermore, a lack of consensus in the field as to what attributes make a gene truly essential adds another aspect of variation. Thus, a universal minimal genome remains elusive. Here, as an alternative to defining a minimal genome, we propose that the concept of gene persistence can be used to classify genes needed for robust long-term survival. Persistent genes, although not ubiquitous, are conserved in a majority of genomes, tend to be expressed at high levels, and are frequently located on the leading DNA strand. These criteria impose constraints on genome organization, and these are important considerations for engineering cells and for creating cellular life-like forms in SB.

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Language(s): eng - English
 Dates: 2012-122013-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.tig.2012.11.001
ISSN: 0168-9525
 Degree: -

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Title: Trends in Genetics
  Other : Trends Genet.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 29 (5) Sequence Number: - Start / End Page: 273 - 279 Identifier: ISSN: 0168-9525
CoNE: https://pure.mpg.de/cone/journals/resource/954925485719