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  4Pi-confocal imaging in fixed biological specimens.

Schrader, M., Bahlmann, K., Giese, G., & Hell, S. W. (1998). 4Pi-confocal imaging in fixed biological specimens. Biophysical Journal, 75(4), 1659-1668. doi:10.1016/S0006-3495(98)77608-8.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-A44D-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-DF65-4
Genre: Journal Article

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BiophysJ_75_1998_1659.pdf (Any fulltext), 2MB
 
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 Creators:
Schrader, Martin, Author
Bahlmann, Karsten, Author
Giese, Günter1, Author              
Hell, S. W.2, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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 Abstract: By combining the wavefronts produced by two high-aperture lenses, two-photon 4Pi-confocal microscopy allows three-dimensional imaging of transparent biological specimens with axial resolution in the 100–140-nm range. We reveal the imaging properties of a two-photon 4Pi-confocal microscope as applied to a fixed cell. We demonstrate that a fast, linear point deconvolution suffices to achieve axially superresolved 3D images in the cytoskeleton. Furthermore, we describe stringent algorithms for alignment and control of the two lenses. We also show how to compensate for the effects of a potential refractive index mismatch of the mounting medium with respect to the immersion system.

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Language(s): eng - English
 Dates: 1998-03-021998-06-222008-11-241998-10
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 75 (4) Sequence Number: - Start / End Page: 1659 - 1668 Identifier: Other: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117