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  Electroporation of nucleic acids into prokaryotic and eukaryotic cells by square wave pulses

Hänze, J., Fischer, L., Koenen, M., Worgall, S., & Rascher, W. (1998). Electroporation of nucleic acids into prokaryotic and eukaryotic cells by square wave pulses. Biotechnology Techniques, 12(2), 159-163. doi:10.1023/A:1008800903452.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-A4CF-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-A260-1
Genre: Journal Article

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BiotechnolTechn_12_1998_159.pdf (Any fulltext), 187KB
 
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 Creators:
Hänze, Jörg, Author
Fischer, Lothar, Author
Koenen, Michael1, 2, 3, 4, Author              
Worgall, Stefan, Author
Rascher, Wofgang, Author
Affiliations:
1Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society, ou_1497727              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
3Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society, ou_1497748              
4Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: A novel electroporator is presented that generates adjustable square wave pulses with a maximal pulse strength of 2500 V / 100 A and a pulse length between 0.1 and 15 ms and that efficiently transfects prokaryotic and eukaryotic cells. Various factors influencing DNA transfer into tissue culture cells, embryonic stem cells and E. coli K12 cells were optimized and the respective electrical pulse profiles monitored.

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Language(s): eng - English
 Dates: 1997-12-051997-10-271997-12-241998-02
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666620
DOI: 10.1023/A:1008800903452
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Title: Biotechnology Techniques
  Alternative Title : Biotechnology Techniques
Source Genre: Journal
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Publ. Info: Dordrecht [u.a.] : Springer Science + Business Media B.V
Pages: - Volume / Issue: 12 (2) Sequence Number: - Start / End Page: 159 - 163 Identifier: ISSN: 1573-6784