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  A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine

Fakler, B., Brändle, U., Bond, C. T., Glowatzki, E., König, C., Adelman, J. P., et al. (1994). A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine. FEBS Letters, 356(2), 199-203. doi:10.1016/0014-5793(94)01258-X.

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FEBSLett_356_1994_199.pdf (Any fulltext), 461KB
 
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 Creators:
Fakler, Bernd, Author
Brändle, Uwe, Author
Bond, Chris T., Author
Glowatzki, Elisabeth, Author
König, C., Author
Adelman, John P., Author
Zenner, Hans−Peter, Author
Ruppersberg, J. Peter1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: Inward rectifier; K+ channel; Clone; Spermine; Site-directed mutagenesis
 Abstract: Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.

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Language(s): eng - English
 Dates: 1994-10-311994-11-071994-12-19
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666748
DOI: 10.1016/0014-5793(94)01258-X
URI: https://www.ncbi.nlm.nih.gov/pubmed/7805837
Other: 4107
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 356 (2) Sequence Number: - Start / End Page: 199 - 203 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501