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  Modulation of Ca2+ channels by PTX-sensitive G-proteins is blocked by N- ethylmaleimide in rat sympathetic neurons

Shapiro, M. S., Wollmuth, L. P., & Hille, B. (1994). Modulation of Ca2+ channels by PTX-sensitive G-proteins is blocked by N- ethylmaleimide in rat sympathetic neurons. The Journal of Neuroscience, 14(11), 7109-7116. Retrieved from http://www.jneurosci.org/content/14/11/7109.

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Shapiro, M. S., Author
Wollmuth, Lonnie P.1, Author           
Hille, Bertil, Author
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1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: G-proteins; CA2+ Channels; N-ethylmaleimide; signal transduction; sympathetic neurons
 Abstract: The actions of N-ethylmaleimide (NEM), a sulfhydryl alkylating agent, on G-protein-mediated inhibition of N-type Ca2+ channels in adult rat superior cervical ganglion (SCG) neurons were studied using whole-cell voltage clamp. In SCG neurons, inhibition of ICa occurs by at least three separable pathways: one pertussis toxin (PTX) sensitive and voltage dependent, and two PTX insensitive and voltage independent. NEM blocked PTX-sensitive inhibition nearly completely, with only small effects on PTX-insensitive inhibition. Somatostatin inhibition is completely PTX sensitive and was wholly blocked by a 120 sec exposure to 50 microM NEM, with shorter exposure times producing a less complete block. Inhibition of ICa by norepinephrine (NE) is approximately half PTX sensitive and was also approximately half NEM sensitive. One component of muscarinic inhibition is PTX insensitive, voltage independent, and mediated by a diffusible cytoplasmic messenger; this pathway was largely spared by NEM treatment. Another pathway is also PTX insensitive and voltage independent, used by substance P, and was also largely NEM insensitive. Hence, in SCG neurons, NEM selectively inactivates PTX-sensitive G-proteins. We also find evidence that the PTX-insensitive action of NE is distinct from the other PTX-insensitive pathways, and therefore assign it to a fourth signaling pathway.

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Language(s): eng - English
 Dates: 1994-11-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: The Journal of Neuroscience
  Other : The Journal of Neuroscience: the Official Journal of the Society for Neuroscience
  Abbreviation : J. Neurosci.
Source Genre: Journal
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Publ. Info: Washington, DC : Society of Neuroscience
Pages: - Volume / Issue: 14 (11) Sequence Number: - Start / End Page: 7109 - 7116 Identifier: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1