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  Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy

Denk, W., Delaney, K. R., Gelperin, A., Kleinfeld, D., Strowbridge, B. W., Tank, D. W., et al. (1994). Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy. Journal of Neuroscience Methods, 54(2), 151-162. doi:10.1016/0165-0270(94)90189-9.

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JNeurosciMeth_54_1994_151.pdf (Any fulltext), 2MB
 
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Denk, Winfried1, Author           
Delaney, Kerry R., Author
Gelperin, Alan, Author
Kleinfeld, David, Author
Strowbridge, B. W., Author
Tank, David W., Author
Yuste, Rafael, Author
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: Microscopy, 2-photon laser scanning; Light scattering; Phototoxicity; Receptor, neurotransmitter
 Abstract: Light scattering by brain tissue and phototoxicity are major obstacles to the use of high-resolution optical imaging and photo-activation ('uncaging') of bioactive compounds from inactive ('caged') precursors in intact and semi-intact nervous systems. Optical methods based on 2-photon excitation promise to reduce these obstacles (Denk, 1994; Denk et al., 1990, 1994). Here we show a range of imaging modes based on 2-photon laser scanning microscopy (TPLSM) as applicable to problems in neuroscience. Fluorescence images were taken of neurons labeled with ion-sensitive and voltage-sensitive dyes in invertebrate ganglia, mammalian brain slices, and from the intact mammalian brain. Scanning photochemical images with whole-cell current detection (Denk, 1994) show how the distribution of neurotransmitter receptors on the surface of specific cells can be mapped. All images show strong optical sectioning and usable images can be obtained at depths greater than 100 microns below the surface of the preparation.

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Language(s): eng - English
 Dates: 1994-06-281994-06-282003-03-181994-10
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of Neuroscience Methods
  Other : J. Neurosci. Meth.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 54 (2) Sequence Number: - Start / End Page: 151 - 162 Identifier: ISSN: 0165-0270
CoNE: https://pure.mpg.de/cone/journals/resource/954925480594