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  Kindling increases N-methyl-d-aspartate potency at single N-methyl-d-aspartate channels in dentate gyrus granule cells

Köhr, G., & Mody, I. (1994). Kindling increases N-methyl-d-aspartate potency at single N-methyl-d-aspartate channels in dentate gyrus granule cells. Neuroscience, 62(4), 975-981. doi:10.1016/0306-4522(94)90336-0.

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Köhr, Georg1, 2, 3, Author              
Mody, Istvan, Author
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1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Directly responsible to the Managing Director, Max Planck Institute for Medical Research, Max Planck Society, ou_persistent22              
3Georg Köhr Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497714              

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Free keywords: d-AP5D; (−)-2-amino-5-phosphonopentanoic acid; NMDA; N-methyl-d-aspartate
 Abstract: Dose-response studies of N-methyl-D-aspartate channel openings were carried out using cell-attached patches in dentate gyrus granule cells acutely isolated from control and kindled rats. The tips of the patch electrodes were first filled with regular extracellular solution, followed by backfilling through the shank with the agonist containing solution. As the two solutions joined, the agonist (N-methyl-D-aspartate, 25 microM) steadily diffused to the cell membrane, and the concentration gradually built up resulting in the progressive increase in the opening probability of N-methyl-D-aspartate channels. The reliability of this cell-attached diffusional drug delivery method was tested by determining the concentration dependence of competitive antagonism of N-methyl-D-aspartate induced channel activity by D(-)-2-amino-5-phosphonopentanoic acid. The Ki for D(-)-2-amino-5-phosphonopentanoic acid in the presence of 25 microM N-methyl-D-aspartate was found to be 6.8 microM. Twenty-four hours following the last seizure, N-methyl-D-aspartate channels on kindled neurons were consistently activated by lower N-methyl-D-aspartate concentrations than channels on control granule cells, indicating a higher potency of agonist at epileptic N-methyl-D-aspartate channels. The higher potency of the agonist is most likely a reflection of the long-term alterations in the modulation of N-methyl-D-aspartate receptor function in epileptic neurons.

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Language(s): eng - English
 Dates: 1994-07-122003-03-141994-10
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666768
DOI: 10.1016/0306-4522(94)90336-0
URI: https://www.ncbi.nlm.nih.gov/pubmed/7531306
Other: 4114
 Degree: -

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Title: Neuroscience
Source Genre: Journal
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Publ. Info: Oxford : Pergamon
Pages: - Volume / Issue: 62 (4) Sequence Number: - Start / End Page: 975 - 981 Identifier: ISSN: 0306-4522
CoNE: https://pure.mpg.de/cone/journals/resource/954925514498