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キーワード:
cardiac muscarinic receptors; phospholipase A2; sarcolemma; quinuclidinyl benzilate; antagonist binding; binding kinetics
要旨:
Activation of phospholipases during prolonged myocardial ischemia could contribute to the functional derangement of myocardial cells by altering the phospholipid environment of a number of membrane bound proteins including receptors. The present study examined the kinetics of muscarinic receptor antagonist [3H] quinuclidinyl benzilate binding ([3H]QNB) to muscarinic receptors of highly purified sarcolemmal membranes under control conditions and after treatment with phospholipase A2 (PLA2; EC 3.1.1.4.). Initial binding rates of QNB exhibited saturation kinetics, when plotted against the ligand concentration in control and PLA2 treated sarcolemmal membranes. This kinetic behaviour of QNB−binding is consistent with at least a two step binding mechanism. According to this two step binding hypothesis an unstable intermediate receptor−QNB complex (R*QNB) forms rapidly, and this form undergoes a slow conversion to the high affinity ligand−receptor complex R−QNB. The Michaelis constant Km of R−QNB formation was 1.8 nM, whereas the dissociation constant Kd obtained from equilibrium measurements was 0.062 nM. After 5 min exposure of sarcolemmal membranes to PLA2QNB binding capacity (Bmax) was reduced by 62%, and the affinity of the remaining receptor sites was decreased by 47% (Kd = 0.116 nM). This PLA2−induced increase of Kd was accompanied by a corresponding increase of Km, whereas the rate constants k2 and k−2 of the hypothetical slow conversion step (second reaction step) remained unchanged. These results suggest that binding of QNB to cardiac muscarinic receptors induces a transition in the receptor−ligand configuration, which is necessary for the formation of the final high affinity R−QNB complex. PLA2−induced changes of the lipid environment result in the inability of a part of the receptor population to undergo this transition, thereby inhibiting high affinity QNB−binding
