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  Small N-Terminal Deletion by Splicing in Cerebellar α6 Subunit Abolishes GABAA Receptor Function

Korpi, E. R., Kuner, T., Kristo, P., Köhler, M., Herb, A., Lüddens, H., et al. (1994). Small N-Terminal Deletion by Splicing in Cerebellar α6 Subunit Abolishes GABAA Receptor Function. Journal of Neurochemistry: official journal of the International Society for Neurochemistry, 63(3), 1167-1170. doi:10.1046/j.1471-4159.1994.63031167.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-A8D8-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-AFF9-3
Genre: Journal Article

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JNeurochem_63_1994_1167.pdf (Any fulltext), 426KB
 
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 Creators:
Korpi, Esa R., Author
Kuner, Thomas1, 2, 3, 4, 5, Author              
Kristo, P., Author
Köhler, Martin4, Author              
Herb, Anne4, Author              
Lüddens, Hartmut, Author
Seeburg, Peter H.4, Author              
Affiliations:
1Interdisciplinary WIN-Research Group on Olfactory Dynamics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497717              
2Synaptic Transmission MNTB, Max Planck Institute for Medical Research, Max Planck Society, ou_1497745              
3Synaptic Transmission, Max Planck Institute for Medical Research, Max Planck Society, ou_1497744              
4Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
5Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: γ-Aminobutyric acidA receptor; Splicing; Cerebellum; Recombinant receptors; In situ hybridization
 Abstract: Sequence variation was found in cDNA coding for the extracellular domain of the rat γ-aminobutyric acid type A (GABAA) receptor α6 subunit. About 20% of polymerase chain reaction (PCR)-amplified α6 cDNA prepared from rat cerebellar mRNA lacked nucleotides 226–255 as estimated by counting single-stranded phage plaques hybridized specifically to the short (α6S) and long (wild-type) forms of the α6 mRNA. Genomic PCR revealed an intron located upstream of the 30-nucleotide sequence. Both splice forms were detected in the cerebellum by in situ hybridization. Recombinant receptors, resulting from coexpression of the α6S subunit with the GABAA receptor β2 and γ2 subunits in human embryonic kidney 293 cells, were inactive at binding [3H]muscimol and [3H]Ro 15-4513. In agreement, injection of complementary RNAs encoding the same subunits into Xenopus oocytes produced only weak GABA-induced currents, indistinguishable from those produced by β2γ2 receptors. Therefore, the 10 amino acids encoded by the 30-nucleotide fragment may be essential for the correct assembly or folding of the α6 subunit-containing receptors.

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Language(s): eng - English
 Dates: 1994-05-201994-05-202002-11-231994-09
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 666771
DOI: 10.1046/j.1471-4159.1994.63031167.x
Other: 4085
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Title: Journal of Neurochemistry : official journal of the International Society for Neurochemistry
  Other : J. Neurochem.
Source Genre: Journal
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Publ. Info: New York : Raven Press [etc.]
Pages: - Volume / Issue: 63 (3) Sequence Number: - Start / End Page: 1167 - 1170 Identifier: ISSN: 0022-3042
CoNE: https://pure.mpg.de/cone/journals/resource/954925416956