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  Somatic cell genetic and biochemical characterization of cell lines resulting from human genomic DNA transfections of Chinese hamster ovary cell mutants defective in sterol-dependent activation of sterol synthesis and LDL receptor expression

Hasan, M. T., Chang, C. C. Y., & Chang, T. (1994). Somatic cell genetic and biochemical characterization of cell lines resulting from human genomic DNA transfections of Chinese hamster ovary cell mutants defective in sterol-dependent activation of sterol synthesis and LDL receptor expression. Somatic Cell and Molecular Genetics, 20(3), 183-194. doi:10.1007/BF02254759.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-A922-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-B1CD-9
Genre: Journal Article

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Hasan, Mazahir T.1, 2, 3, Author              
Chang, Catherine C. Y., Author
Chang, Ta−Yuan, Author
Affiliations:
1Mazahir Hasan Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497726              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
3Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: We have isolated several non-leaky mutant Chinese hamster ovary (CHO) cell clones (M4, M19, and M21) requiring cholesterol and unsaturated fatty acid for growth. These mutants belong to the same complementation group as the mutant M1 cells previously reported from this laboratory. M19 cells reverted to lipid prototrophy at very low frequency and were chosen as recipients to perform DNA-mediated gene-transfer experiments using total human genomic DNAs. Biochemical characterization of these transfectant clones indicated that, unlike their parental M19 cells, they were able to exhibit activation of cholesterol biosynthesis and LDL receptor expression in response to sterol removal from the growth medium. RNA blotting analysis indicated that these transfectants were able to increase HMG-CoA synthase gene transcripts in response to sterol removal. From the genomic DNAs of a representative secondary transfectant cells, we cloned a unique human DNA fragment (designated as h lambda 2) and showed that h lambda 2 closely linked with the presumptive human M1 gene.

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Language(s): eng - English
 Dates: 1994-03-011994-04-281994-05
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 664707
DOI: 10.1007/BF02254759
URI: https://www.ncbi.nlm.nih.gov/pubmed/7940020
Other: 7431
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Title: Somatic Cell and Molecular Genetics
  Other : Somatic Cell and Molecular Genetics
Source Genre: Journal
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Publ. Info: New York, NY : Kluwer Academic Publishers
Pages: - Volume / Issue: 20 (3) Sequence Number: - Start / End Page: 183 - 194 Identifier: ISSN: 0740-7750
CoNE: https://pure.mpg.de/cone/journals/resource/954927608797