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  High resolution analysis of cell cycle-correlated vimentin expression in asynchronously grown, TPA-treated MPC-11 cells by the novel flow cytometric multiparameter BrdU-hoechst/PI and immunolabeling technique

Giese, G., Kubbies, M., & Traub, P. (1994). High resolution analysis of cell cycle-correlated vimentin expression in asynchronously grown, TPA-treated MPC-11 cells by the novel flow cytometric multiparameter BrdU-hoechst/PI and immunolabeling technique. Journal of Cellular Physiology, 161(2), 209-216. doi:10.1002/jcp.1041610204.

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JCellPhysiology_161_1994_209.pdf (Any fulltext), 835KB
 
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Giese, Günter1, 2, Author              
Kubbies, Manfred, Author
Traub, Peter3, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Light Microscopy Facility, Max Planck Institute for Medical Research, Max Planck Society, ou_1497720              
3Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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 Abstract: High resolution, multiparameter analysis using the flow cytometric BrdU/Hoechst quenching technique has been applied to study cell cycle kinetics and vimentin expression in individual cells of asynchronously grown MPC−11 mouse plasmacytoma cell cultures treated with 12−O−tetradecanoylphorbol−13−acetate (TPA) to induce in vitro differentiation. BrdU treatment up to 16 h in the absence or presence of TPA did not affect either cell cycle progression or the kinetics or quantity of vimentin expression. TPA−treated cells became arrested in G1 phase of the second cell cycle; however, this G1 phase arrest was transient only. In addition, G1 phase cells located prior to a putative transition point at the beginning of TPA treatment were completely blocked in cell cycle progression. There is also evidence that cells located in G1 or G2/M phase at the beginning of TPA treatment finally expressed low levels of vimentin. On the contrary, cells located in S phase at TPA exposure showed high vimentin levels after treatment. The results presented here show that, with the flow cytometric BrdU/Hoechst quenching technique, one can correlate time−dependent protein expression at the single cell level in asynchronously grown cultures not only with the actual cell cycle state, but also with the history of cell replication

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Language(s): eng - English
 Dates: 1993-09-071994-04-251994-11-01
 Publication Status: Published in print
 Pages: 8
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 Rev. Type: Peer
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Title: Journal of Cellular Physiology
  Other : J. Cell. Physiol.
Source Genre: Journal
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Publ. Info: Hoboken, NJ : Wiley Subscription Services, Inc.
Pages: - Volume / Issue: 161 (2) Sequence Number: - Start / End Page: 209 - 216 Identifier: ISSN: 0021-9541
CoNE: https://pure.mpg.de/cone/journals/resource/110992357271180