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  Stable expression of cloned rat GABAA receptor subunits in a human kidney cell line

Hamilton, B. J., Lennon, D. J., Im, H. K., Im, W. B., Seeburg, P. H., & Carter, D. B. (1993). Stable expression of cloned rat GABAA receptor subunits in a human kidney cell line. Neuroscience Letters, 153(2), 206-209. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/7687050.

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Genre: Journal Article
Alternative Title : Stable expression of cloned rat GABAA receptor subunits in a human kidney cell line

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NeurosciLett_153_1993_206.pdf (Any fulltext), 288KB
 
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Hamilton, B. J., Author
Lennon, D. J., Author
Im, H. K., Author
Im, W. B., Author
Seeburg, Peter H.1, Author           
Carter, D. B., Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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 Abstract: A predominant form of the GABAA/benzodiazepine receptor-Cl- channel complex is believed to consist of three different 48-55 kDa subunits (alpha, beta, gamma) with unknown stoichiometry. Plasmids containing the rat GABAA receptor cDNAs coding for alpha 1, beta 2, and gamma 2 were co-transfected, along with a plasmid encoding G418 resistance, into human embryonic kidney cells previously transformed with Adenovirus 5 (HEK-293) [J. Gen. Virol., 36 (1977) 59-72]. Four percent of the G418 resistant colonies were found to express mRNA for all three of the GABAA subunits constitutively. A single cell clone derived from one of the alpha 1 beta 2 gamma 2 expressors has demonstrated stable electrophysiological characteristics over 25 passages. The GABA-activated Cl- current in this cell line is blocked by picrotoxin and bicuculline, and is modulated by a variety of agonist and inverse agonist ligands including diazepam, Ro 154513, zolpidem, and beta-CCE. The cell line has been used successfully over a 12-month period as a screen for novel drugs modulating GABA-mediated polarization of neuronal cells.

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Language(s): eng - English
 Dates: 1993-01-211992-05-111993-01-211993-04-30
 Publication Status: Issued
 Pages: 4
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666787
URI: https://www.ncbi.nlm.nih.gov/pubmed/7687050
Other: 4052
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Title: Neuroscience Letters
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 153 (2) Sequence Number: - Start / End Page: 206 - 209 Identifier: ISSN: 0304-3940
CoNE: https://pure.mpg.de/cone/journals/resource/954925512448