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  The calcium signal in human neutrophils and its relation to exocytosis investigated by patch-clamp capacitance and Fura-2 measurements

Nüße, O., & Lindau, M. (1993). The calcium signal in human neutrophils and its relation to exocytosis investigated by patch-clamp capacitance and Fura-2 measurements. Cell Calcium, 14(4), 255-269. doi:10.1016/0143-4160(93)90047-A.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-AA89-E Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-1EFF-7
Genre: Journal Article
Alternative Title : The calcium signal in human neutrophils and its relation to exocytosis investigated by patch-clamp capacitance and Fura-2 measurements

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 Creators:
Nüße, Oliver, Author
Lindau, Manfred1, Author           
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: Intracellular calcium ([Ca2+]i) and exocytosis of human neutrophils were investigated with patch-clamp capacitance and Fura-2 fluorescence measurements. Intracellular application of GTPγS induces a calcium transient and exocytosis. The onset of degranulation occurs at the time where the maximal [Ca2+]i is reached. Despite the close correlation in time, buffering [Ca2+]i at the resting level or at ∼2 μM leaves the extent and the time course of degranulation unchanged. The decay of the calcium transient is due to diffusional equilibration between the cytosol and the pipette volume. GTPγS activates no cellular mechanisms for Ca2+ reuptake or extrusion. The endogenous calcium buffer capacity can be estimated to be as low as that of ∼90 μM Fura-2. Stimulation with fMLP also induces degranulation and a calcium transient. The decay of fMLP-induced calcium transients is much faster than that of GTPγS-induced transients and is independent of diffusion indicating that fMLP also induces rapid reuptake or extrusion of Ca2+. Degranulation but not the calcium transient requires the presence of intracellular GTP. Different signalling pathways appear to be involved in GTPγS- and fMLP-stimulated calcium signals. The intracellular calcium release is not an essential signal to initiate exocytosis in neutrophils.

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Language(s): eng - English
 Dates: 1992-07-081992-08-211993-04-01
 Publication Status: Issued
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665071
DOI: 10.1016/0143-4160(93)90047-A
Other: 7014
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Title: Cell Calcium
Source Genre: Journal
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Publ. Info: Amsterdam, Netherlands : Churchill Livingstone
Pages: - Volume / Issue: 14 (4) Sequence Number: - Start / End Page: 255 - 269 Identifier: ISSN: 0143-4160
CoNE: https://pure.mpg.de/cone/journals/resource/954922647086