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  Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy

Stuart, G., Dodt, H.-U., & Sakmann, B. (1993). Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflügers Archiv: European Journal of Physiology, 423(5), 511-518. doi:10.1007/BF00374949.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-AADC-3 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-163C-1
Genre: Journal Article
Alternative Title : Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy

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PflügArch_423_1993_511.pdf (Any fulltext), 3MB
 
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http://link.springer.com/content/pdf/10.1007%2FBF00374949.pdf (Any fulltext)
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 Creators:
Stuart, Greg1, Author           
Dodt, H.-U., Author
Sakmann, Bert1, Author           
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: A description is given of the implementation of infrared differential interference contrast (IR-DIC) video microscopy to an upright compound microscope. Using the improved resolution offered by IR-DIC a procedure is described for making patch-pipette recordings from visually identified neuronal somata and dendrites in brain slices. As an example of the application of this technique to electrophysiological recordings from small neuronal processes in brain slices we describe whole-cell current-clamp and cell-attached and excised patch-clamp recordings from the apical dendrites of layer V pyramidal neurons in slices of rat neocortex.

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Language(s): eng - English
 Dates: 1993-01-041993-01-191993-06-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666792
DOI: 10.1007/BF00374949
URI: https://www.ncbi.nlm.nih.gov/pubmed/8351200
Other: 4046
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Title: Pflügers Archiv: European Journal of Physiology
  Other : Pflügers Arch. Europ. J. Physiol.
Source Genre: Journal
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Publ. Info: Heidelberg : Springer-Verlag
Pages: - Volume / Issue: 423 (5) Sequence Number: - Start / End Page: 511 - 518 Identifier: ISSN: 0031-6768
CoNE: https://pure.mpg.de/cone/journals/resource/954925432380