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  Time-resolved crystallography on H-ras p21

Scheidig, A. J., Pai, E. F., Schlichting, I., Corrie, J. E. T., Reid, G. P., Wittinghofer, A., et al. (1992). Time-resolved crystallography on H-ras p21. Philosophical Transactions of the Royal Society A, 340(1657), 263-272. Retrieved from https://www.jstor.org/stable/53892?seq=1#metadata_info_tab_contents.

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 Creators:
Scheidig, Axel J.1, Author              
Pai, Emil F.1, Author              
Schlichting, Ilme1, 2, Author              
Corrie, John E. T., Author
Reid, Gordon P., Author
Wittinghofer, Alfred1, Author              
Goody, Roger S.1, Author              
Liljas, A., Author
Moffat, K., Author
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Photoreceptors, Max Planck Institute for Medical Research, Max Planck Society, ou_1856341              

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 Abstract: We describe here the results obtained to date on a project aimed at characterizing the changes occurring in the protein product (p21) of the H-ras proto-oncogene during and as a result of hydrolysis of GTP at its active site. The approach used involves crystallization of p21 with a photosensitive precursor of GTP (caged GTP) at the active site followed by generation of GTP by photolysis and collection of X-ray diffraction data using the Laue method at a synchrotron source. The structure of p21 complexed with a single diastereomer of caged GTP is presented here. In contrast to crystals obtained with mixed diastereomers, the nucleotide appears to bind in a manner which is very similar to that of other guanine nucleotides (GDP, GTP, GppNHp). The current state of time resolved structural experiments using these crystals is presented.

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Language(s): eng - English
 Dates: 1992-08-15
 Publication Status: Published in print
 Pages: 12
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 Table of Contents: -
 Rev. Type: Peer
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Title: Philosophical Transactions of the Royal Society A
  Other : Phil. Trans. R. Soc. A
Source Genre: Journal
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Publ. Info: London : Royal Society
Pages: - Volume / Issue: 340 (1657) Sequence Number: - Start / End Page: 263 - 272 Identifier: ISSN: 1364-503X
CoNE: https://pure.mpg.de/cone/journals/resource/954928604111_3