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  Derivatization of ribosomes and of tRNA with an undecagold cluster: crystallographic and functional studies

Weinstein, S., Jahn, W., Laschever, M., Arad, T., Tichelaar, W., Haider, M., et al. (1992). Derivatization of ribosomes and of tRNA with an undecagold cluster: crystallographic and functional studies. Journal of Crystal Growth, 122(1), 286-292. doi:10.1016/0022-0248(92)90257-J.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-AB45-B Version Permalink: https://hdl.handle.net/21.11116/0000-0002-5E0D-8
Genre: Journal Article

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JCrystGrowth_122_1992_286.pdf (Any fulltext), 2MB
 
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 Creators:
Weinstein, S., Author
Jahn, Werner1, Author           
Laschever, M., Author
Arad, T., Author
Tichelaar, Willem2, Author           
Haider, M., Author
Glotz, C., Author
Boeckh, T., Author
Berkovitch-Yellin, Z., Author
Franceschi, F., Author
Yonath, A., Author
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: An undecagold cluster was covalently attached to whole ribosomes and to their small and large subunits prior to their crystallization. X-ray crystallographic data were collected from crystals of the first two. The same cluster was bound to tRNAphe fromE. coli at base 47. It was found that the modified tRNA molecule binds to the ribosome and can be aminoacylated by its cognate synthetase. The gold cluster modified tRNAphe may be used for phasing diffraction data of crystals of complexes containing it, mimicking defined states in the process of protein biosynthesis.

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Language(s): eng - English
 Dates: 2002-07-301992-08-01
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0022-0248(92)90257-J
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Title: Journal of Crystal Growth
  Abbreviation : J. Cryst. Growth
Source Genre: Journal
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Publ. Info: Amsterdam : North-Holland
Pages: - Volume / Issue: 122 (1) Sequence Number: - Start / End Page: 286 - 292 Identifier: ISSN: 0022-0248
CoNE: https://pure.mpg.de/cone/journals/resource/954925412860