English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse
  1. Item Summary / 
  2. View item

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells

Metzger, M., Bellemann, P., Schwartz, T., & Geider, K. (1992). Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells. Nucleic Acids Research (London), 20(9), 2265-2270. doi:10.1093/nar/20.9.2265.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-AB81-5 Version Permalink: https://hdl.handle.net/21.11116/0000-0002-5FA2-D
Genre: Journal Article

Files

show Files
hide Files
:
NuclAcidRes_20_1992_2265.pdf (Any fulltext), 5MB
 
File Permalink:
-
Name:
NuclAcidRes_20_1992_2265.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://academic.oup.com/nar/article-pdf/20/9/2265/3786545/20-9-2265.pdf (Any fulltext)
Description:
-
OA-Status:
Locator:
https://doi.org/10.1093/nar/20.9.2265 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Metzger, Marianne1, Author           
Bellemann, Peter1, Author           
Schwartz, Thomas2, Author           
Geider, Klaus1, Author           
Affiliations:
1Research Group Prof. Dr. Geider, Max Planck Institute for Medical Research, Max Planck Society, ou_1497738              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

Content

show
hide
Free keywords: -
 Abstract: The suicide plasmid pfdA31-Tn5 was constructed to mutagenize Erwinia amylovora and Escherichia coli strains by electorporation. This vector carries the bacteriophage fd replication origin, a beta-lactamase gene and the transposon Tn5. For propagation the plasmid depends on host cells producing fd gene-2 protein. Electroporation of E.amylovora or E.coli cells with plasmid pfdA31-Tn5 yielded more than 10(4) transposition events per micrograms DNA. We have produced and characterized transposon mutants of E.amylovora affecting either galactose metabolism or the synthesis of the phytotoxin (L)-2,5-dihydrophenylalanine. A Tn5-insertion in a gene, involved in exopolysaccharide synthesis of E.amylovora strain Ea7/74, was subcloned into vector pfdA31 and used to mutagenize E.amylovora strain Ea1/79 by site-directed recombination.

Details

show
hide
Language(s): eng - English
 Dates: 1992-03-311992-02-041992-03-311992-05-11
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1093/nar/20.9.2265
URI: https://www.ncbi.nlm.nih.gov/pubmed/1317549
URI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC312340/
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic Acids Research (London)
  Other : Nucleic Acids Res
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press
Pages: - Volume / Issue: 20 (9) Sequence Number: - Start / End Page: 2265 - 2270 Identifier: ISSN: 0305-1048
CoNE: https://pure.mpg.de/cone/journals/resource/110992357379342