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  Different mechanisms regulate muscle-specific AChR gamma- and epsilon-subunit gene expression

Numberger, M., Dürr, I., Kues, W., Koenen, M., & Witzemann, V. (1991). Different mechanisms regulate muscle-specific AChR gamma- and epsilon-subunit gene expression. EMBO Journal, 10(10), 2957-2964. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/1655408.

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 Creators:
Numberger, Markus1, Author              
Dürr, Ingolf, Author
Kues, Winfried1, Author              
Koenen, Michael1, 2, 3, 4, Author              
Witzemann, Veit1, 2, 3, 4, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society, ou_1497727              
3Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
4Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society, ou_1497748              

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 Abstract: Five different subunits, alpha, beta, gamma, delta and epsilon, constitute the acetylcholine receptors from mammalian skeletal muscle. Their corresponding mRNA levels are regulated differentially. In particular, mRNAs encoding the gamma- and epsilon-subunits, which specify two AChR isoforms, show a reciprocal behaviour during synapse formation and maturation. We have isolated 5' flanking sequences of the gamma- and epsilon-subunit genes that confer muscle-specific expression upon transient transfection of primary cultures of rat muscle cells. The gamma-subunit gene fragment contains two adjacent CANNTG sequence motifs that are essential for muscle-specific transcriptional activity suggesting transactivation by helix-loop-helix proteins. The epsilon-subunit gene fragment carries only a single CANNTG consensus motif which is not required for expression in transfected muscle cells. This sequence motif is, however, necessary to repress transcriptional activity in non-muscle cells and thus may control the muscle-specific expression of the epsilon-subunit gene. The results suggest that CANNTG motifs together with their 3' and 5' flanking nucleotides provide binding sites for both activating as well as repressing trans-acting factors. These elements could thus contribute to the muscle-specific expression of AChR subunit genes.

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Language(s): eng - English
 Dates: 1991-05-311991-04-091991-10
 Publication Status: Published in print
 Pages: 8
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 Rev. Type: Peer
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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 10 (10) Sequence Number: - Start / End Page: 2957 - 2964 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061