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  Structural determinants of ion flow through recombinant glutamate receptor channels

Verdoorn, T. A., Burnashev, N., Monyer, H., Seeburg, P. H., & Sakmann, B. (1991). Structural determinants of ion flow through recombinant glutamate receptor channels. Science, 252(5013), 1715-1718. doi:10.1126/science.1710829.

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Verdoorn, Todd A., Author
Burnashev, Nail1, Author           
Monyer, Hannah2, Author           
Seeburg, Peter H.2, Author           
Sakmann, Bert1, Author           
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1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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 Abstract: Functional glutamate receptor (GluRs) were transiently expressed in cultured mammalian cells from cloned complementary DNAs encoding GluR-A, -B, -C, or -D polypeptides. The steady-state current-voltage (I-V) relations of glutamate- and kainate-induced currents through homomeric channels fell into two classes: channels composed of either the GluR-A, -C, and -D subunits showed doubly rectifying I-V curves, and channels composed of the GluR-B subunits displayed simple outward rectification. The presence of GluR-B subunits in heteromeric GluRs determined the I-V behavior of the resulting channels. Site-directed mutagenesis identified a single amino acid difference (glutamine to arginine) in the putative transmembrane segment TM2 responsible for subunit-specific I-V relationships. The properties of heteromeric wild-type and mutant GluRs revealed that the dominance of GluR-B is due to the arginine residue in the TM2 region.

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Language(s): eng - English
 Dates: 1991-03-081991-04-301991-06-21
 Publication Status: Published in print
 Pages: 5
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.1710829
URI: 10.1126/science.1710829
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Title: Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 252 (5013) Sequence Number: - Start / End Page: 1715 - 1718 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1