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  Functional properties of recombinant rat GABAA receptors depend upon subunit composition

Verdoorn, T. A., Draguhn, A., Ymer, S., Seeburg, P. H., & Sakmann, B. (1990). Functional properties of recombinant rat GABAA receptors depend upon subunit composition. Neuron, 4(6), 919-928. doi:10.1016/0896-6273(90)90145-6.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-AD37-E Version Permalink: http://hdl.handle.net/21.11116/0000-0000-7FCE-B
Genre: Journal Article

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 Creators:
Verdoorn, Todd A., Author
Draguhn, Andreas1, Author              
Ymer, Sanie, Author
Seeburg, Peter H.2, Author              
Sakmann, Bert1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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 Abstract: GABA-gated chloride channels were expressed in human embryonic kidney cells following transfection of cDNAs encoding the alpha 1, beta 2, and gamma 2 subunits of the rat GABAA receptor (GABAR). Functional properties were determined using patch-clamp techniques in the whole-cell and outside-out configurations. Large whole-cell currents were observed in cells expressing the alpha 1 beta 2, alpha 1 gamma 2, and alpha 1 beta 2 gamma 2 subunit combinations. The unique characteristics of GABAR channels consisting of these subunit combinations depended upon the presence or absence of beta 2 and gamma 3 subunits. GABA-activated currents in cells expressing GABARs with the beta 2 subunit desensitized faster and showed greater outward rectification, and the channels had a shorter mean open time than GABARs composed of alpha 1 gamma 2 subunits. When the gamma 2 subunit was present the resulting GABAR channels had a larger conductance. The slope of the concentration-response curve was significantly steeper for GABARs composed of alpha 1 beta 2 gamma 2 subunits compared with GABARs consisting of alpha 1 beta 2 or alpha 1 gamma 2 subunit combinations.

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Language(s): eng - English
 Dates: 1990-01-291990-03-161990-06
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 4 (6) Sequence Number: - Start / End Page: 919 - 928 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565