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  Primary structure and functional expression of the α-, β-, γ-, δ- and ɛ-subunits of the acetylcholine receptor from rat muscle

Witzemann, V., Stein, E., Barg, B., Konno, T., Koenen, M., Kues, W., et al. (1990). Primary structure and functional expression of the α-, β-, γ-, δ- and ɛ-subunits of the acetylcholine receptor from rat muscle. European Journal of Biochemistry, 194(2), 437-448. doi:10.1111/j.1432-1033.1990.tb15637.x.

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Witzemann, Veit1, 2, 3, 4, Author              
Stein, Elke, Author
Barg, Brigitte, Author
Konno, T., Author
Koenen, Michael1, 2, 3, 4, Author              
Kues, Winfried4, Author              
Criado, Manuel, Author
Hofmann, M., Author
Sakmann, Bert4, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society, ou_1497748              
3Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society, ou_1497727              
4Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: The isolation and characterization of five clones carrying sequences of the α-, β-, γ-, δ- and ɛ-subunit precursors of the rat muscle acetylcholine receptor (AChR) are described. The deduced amino acid sequences indicate that these polypeptides contain 457–519 amino acids and reveal the structural characteristics common to subunits of ligand-gated ion channels. The pattern of subunit-specific mRNA levels in rat muscle shows characteristic changes during development and following denervation, suggesting that innervation of muscle reduces the expression of the α-, β- and δ-subunit mRNAs, suppresses the expression of the γ-subunit mRNA, and induces expression of ɛ-subunit mRNA. Subunit-specific cRNAs generated in vitro were injected into Xenopus laevis oocytes, resulting in the assembly of two functionally different AChR channel subtypes. The AChRγ, composed of the α-, β-, γ- and δ-subunits, has functional properties similar to those of the native AChRs in fetal muscle. The AChRɛ, composed of α-, β-, δ- and ɛ-subunits, corresponds to the end-plate channel of the adult muscle. Thus in rat skeletal muscle the motor nerve regulates the expression of two functionally different AChR subtypes with different molecular composition by the differential expression of subunit-specific mRNAs.

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Language(s): eng - English
 Dates: 1990-05-081990-07-312005-03-031990-12
 Publication Status: Published in print
 Pages: 12
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 Rev. Type: Peer
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Title: European Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 194 (2) Sequence Number: - Start / End Page: 437 - 448 Identifier: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040