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  C-terminal truncation of p21H preserves crucial kinetic and structural properties

John, J., Schlichting, I., Schiltz, E., Rösch, P., & Wittinghofer, A. (1989). C-terminal truncation of p21H preserves crucial kinetic and structural properties. The Journal of Biological Chemistry, 264(22), 13086-13092. Retrieved from http://www.jbc.org/content/264/22/13086.abstract.

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 Creators:
John, Jiss1, 2, Author              
Schlichting, Ilme3, 4, Author              
Schiltz, Emile, Author
Rösch, Paul4, Author              
Wittinghofer, Alfred4, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              
3Photoreceptors, Max Planck Institute for Medical Research, Max Planck Society, ou_1856341              
4Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: The human c-Ha-ras protooncogene product p21C was truncated at the C terminus by 23 amino acids. The resulting G-binding domain, p21 (1-166) = p21C', can be crystallized as a complex with the slowly hydrolyzing GTP analogues guanosin-5'-[beta,gamma-imido]triphosphate, guanosin-5'-[beta,gamma-methylene]triphosphate, and guanosin-5'-O-(3-thiotriphosphate). We show here that this protein has biochemical properties very similar to those of the intact protein. Activating mutations in position 12 (Gly12----Val; Gly12----Arg) have the same effect on the properties of the truncated protein as on intact protein. Nuclear magnetic resonance (NMR) measurements show no apparent effect of the C-terminal deletion on the solution structure of p21. This suggests that neither the structure of the G-binding domain nor any of its biochemical properties are markedly influenced by the truncation.

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Language(s): eng - English
 Dates: 1989-02-281989-08-05
 Publication Status: Published in print
 Pages: 7
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 Table of Contents: -
 Rev. Type: Peer
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Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 264 (22) Sequence Number: - Start / End Page: 13086 - 13092 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1