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  Iterative reconstruction of cryo-electron tomograms using nonuniform fast Fourier transforms

Chen, Y., & Förster, F. (2014). Iterative reconstruction of cryo-electron tomograms using nonuniform fast Fourier transforms. JOURNAL OF STRUCTURAL BIOLOGY, 185(3), 309-316. doi:10.1016/j.jsb.2013.12.001.

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 Creators:
Chen, Yuxiang1, Author              
Förster, Friedrich1, Author              
Affiliations:
1Förster, Friedrich / Modeling of Protein Complexes, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565148              

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Free keywords: 3-DIMENSIONAL RECONSTRUCTION; ELECTRON-MICROSCOPY; ALGORITHM; PROJECTIONS; INVERSION; TOOLBOX; ICE; ARTCryoelectron tomography; Three-dimensional reconstruction; Iterative reconstruction; Image processing;
 Abstract: Algorithms for three-dimensional (3D) reconstruction of objects based on their projections are essential in various biological and medical imaging modalities. In cryo-electron tomography (CET) a major challenge for reconstruction is the limited range of projection angles, which manifests itself as a "missing wedge" of data in Fourier space making the reconstruction problem ill-posed. Here, we apply an iterative reconstruction method that makes use of nonuniform fast Fourier transform (NUFFT) to the reconstruction of cryo-electron tomograms. According to several measures the reconstructions are superior to those obtained using conventional methods, most notably weighted backprojection. Most importantly, we show that it is possible to fill in partially the unsampled region in Fourier space with meaningful information without making assumptions about the data or applying prior knowledge. As a consequence, particles of known structure can be localized with higher confidence in cryotomograms and subtomogram averaging yields higher resolution densities. (C) 2013 Elsevier Inc. All rights reserved.

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Language(s): eng - English
 Dates: 2014-03
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000332815400009
DOI: 10.1016/j.jsb.2013.12.001
 Degree: -

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Title: JOURNAL OF STRUCTURAL BIOLOGY
Source Genre: Journal
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Publ. Info: 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA : ACADEMIC PRESS INC ELSEVIER SCIENCE
Pages: - Volume / Issue: 185 (3) Sequence Number: - Start / End Page: 309 - 316 Identifier: ISSN: 1047-8477