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  Probing surface accessibility of proteins using paramagnetic relaxation in solid-state NMR spectroscopy.

Linser, R., Fink, U., & Reif, B. (2009). Probing surface accessibility of proteins using paramagnetic relaxation in solid-state NMR spectroscopy. Journal of the American Chemical Society, 131(38), 13703-13708. doi:10.1021/ja903892j.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0018-EC4F-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C5C4-8
Genre: Journal Article

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2000348.pdf (Publisher version), 2MB
 
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 Creators:
Linser, R.1, Author              
Fink, U., Author
Reif, B., Author
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1Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society, ou_1950286              

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 Abstract: Paramagnetic Relaxation Enhancement (PRE) can be used to accelerate NMR data acquisition by reducing the longitudinal proton relaxation time T1 in the solid state. We show that the presence of paramagnetic compounds in the bulk solvent induces a site-specific relaxation in addition to local dynamics, which is dependent on the surface accessibility of the respective amide proton in the protein. Differentiation between paramagnetic relaxation and dynamics was achieved by a comparison of 1H T1 times obtained from microcrystalline protein samples prepared with different concentrations of the CuII(edta) chelate. We find that relaxation can in addition be mediated by hydroxyl groups, which transfer relaxation by their ability to exchange with the quickly relaxing bulk solvent. Furthermore, relaxation seems to be transferred by water molecules which diffuse into the protein structure and yield an efficient difference PRE in flexible regions of the protein. The experiments are demonstrated using a perdeuterated sample of the α-spectrin SH3 domain, which was microcrystallized from a buffer containing 90% D2O. Deuteration is a prerequisite to avoid spin diffusion which would otherwise compromise site specific resolution.

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Language(s): eng - English
 Dates: 2009-09-082009-09-30
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/ja903892j
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 131 (38) Sequence Number: - Start / End Page: 13703 - 13708 Identifier: -