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  Proton-detected scalar coupling based assignment strategies in MAS solid-state NMR spectroscopy applied to perdeuterated proteins.

Linser, R., Fink, U., & Reif, B. (2008). Proton-detected scalar coupling based assignment strategies in MAS solid-state NMR spectroscopy applied to perdeuterated proteins. Journal of Magnetic Resonance, 193(1), 89-93. doi:10.1016/j.jmr.2008.04.021.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0018-EE69-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-0BF9-A
Genre: Journal Article

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2007698.pdf (Publisher version), 753KB
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 Creators:
Linser, R.1, Author              
Fink, U., Author
Reif, B., Author
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1Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society, ou_1950286              

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Free keywords: Magic angle spinning (MAS); Perdeuteration; Protein resonance assignment; Scalar coupling based magnetization transfers
 Abstract: Assignment of proteins in MAS (magic angle spinning) solid-state NMR relies so far on correlations among heteronuclei. This strategy is based on well dispersed resonances in the 15N dimension. In many complex cases like membrane proteins or amyloid fibrils, an additional frequency dimension is desirable in order to spread the amide resonances. We show here that proton detected HNCO, HNCA, and HNCACB type experiments can successfully be implemented in the solid-state. Coherences are sufficiently long lived to allow pulse schemes of a duration greater than 70 ms before incrementation of the first indirect dimension. The achieved resolution is comparable to the resolution obtained in solution-state NMR experiments. We demonstrate the experiments using a triply labeled sample of the SH3 domain of chicken α-spectrin, which was re-crystallized in H2O/D2O using a ratio of 1/9. We employ paramagnetic relaxation enhancement (PRE) using EDTA chelated CuII to enable rapid data acquisition.

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Language(s): eng - English
 Dates: 2008-05-062008-07
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.jmr.2008.04.021
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Title: Journal of Magnetic Resonance
Source Genre: Journal
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Pages: - Volume / Issue: 193 (1) Sequence Number: - Start / End Page: 89 - 93 Identifier: -