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  The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons

Kulangara, K., Adler, A. F., Wang, H., Chellappan, M., Hammett, E., Yasuda, R., et al. (2014). The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons. Biomaterials, 35(20), 5327-5336. doi:10.1016/j.biomaterials.2014.03.034.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-0064-8 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-00F7-B
Genre: Journal Article
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 Creators:
Kulangara, K., Author
Adler, A. F., Author
Wang, H., Author
Chellappan, M., Author
Hammett, E., Author
Yasuda, R.1, Author
Leong, K. W., Author
Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288              

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 Abstract: Cellular reprogramming holds tremendous potential for cell therapy and regenerative medicine. Recently, fibroblasts have been directly converted into induced neurons (iNs) by overexpression of the neuronal transcription factors Ascl1, Brn2 and Myt1L. Hypothesizing that cell-topography interactions could influence the fibroblast-to-neuron reprogramming process, we investigated the effects of various topographies on iNs produced by direct reprogramming. Final iN purity and conversion efficiency were increased on micrograting substrates. Neurite branching was increased on microposts and decreased on microgratings, with a simplified dendritic arbor characterized by the reduction of MAP2+ neurites. Neurite outgrowth increased significantly on various topographies. DNA microarray analysis detected 20 differentially expressed genes in iNs reprogrammed on smooth versus microgratings, and quantitative PCR (qPCR) confirmed the upregulation of Vip and downregulation of Thy1 and Bmp5 on microgratings. Electrophysiology and calcium imaging verified the functionality of these iNs. This study demonstrates the potential of applying topographical cues to optimize cellular reprogramming.

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 Dates: 2014
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: http://www.ncbi.nlm.nih.gov/pubmed/24709523
DOI: 10.1016/j.biomaterials.2014.03.034
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Title: Biomaterials
Source Genre: Journal
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Publ. Info: Guildford, England : Elsevier
Pages: 9 Volume / Issue: 35 (20) Sequence Number: - Start / End Page: 5327 - 5336 Identifier: ISSN: 0142-9612
CoNE: https://pure.mpg.de/cone/journals/resource/954925472369