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  Phyllotreta striolata flea beetles utilize host plant defense compounds to create their own glucosinolate-myrosinase system

Beran, F., Pauchet, Y., Kunert, G., Reichelt, M., Wielsch, N., Vogel, H., et al. (2014). Phyllotreta striolata flea beetles utilize host plant defense compounds to create their own glucosinolate-myrosinase system. Proceedings of the National Academy of Sciences of the United States of America, 111(20), 7349-7354. doi:10.1073/pnas.1321781111.

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 Creators:
Beran, Franziska1, Author              
Pauchet, Yannick2, Author              
Kunert, Grit3, Author              
Reichelt, Michael3, Author              
Wielsch, Natalie4, Author              
Vogel, Heiko2, Author              
Reinecke, Andreas5, Author              
Svatoš, Aleš4, Author              
Mewis, I., Author
Schmid, D., Author
Ramasamy, S., Author
Ulrichs, C., Author
Hansson, Bill S.5, Author              
Gershenzon, Jonathan3, Author              
Heckel, David G.2, Author              
Affiliations:
1Research Group Dr. F. Beran, Detoxification in Insects, MPI for Chemical Ecology, Max Planck Society, Beutenberg Campus, Hans-Knoell-Strasse 8, 07745 Jena, DE, ou_1933291              
2Department of Entomology, Prof. D. G. Heckel, MPI for Chemical Ecology, Max Planck Society, ou_421895              
3Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
4Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              
5Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421894              

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 Abstract: The ability of a specialized herbivore to overcome the chemical defense of a particular plant taxon not only makes it accessible as a food source but may also provide metabolites to be exploited for communication or chemical defense. Phyllotreta flea beetles are adapted to crucifer plants (Brassicales) that are defended by the glucosinolate-myrosinase system, the so-called "mustard-oil bomb." Tissue damage caused by insect feeding brings glucosinolates into contact with the plant enzyme myrosinase, which hydrolyzes them to form toxic compounds, such as isothiocyanates. However, we previously observed that Phyllotreta striolata beetles themselves produce volatile glucosinolate hydrolysis products. Here, we show that P. striolata adults selectively accumulate glucosinolates from their food plants to up to 1.75% of their body weight and express their own myrosinase. By combining proteomics and transcriptomics, a gene responsible for myrosinase activity in P. striolata was identified. The major substrates of the heterologously expressed myrosinase were aliphatic glucosinolates, which were hydrolyzed with at least fourfold higher efficiency than aromatic and indolic glucosinolates, and β-O-glucosides. The identified beetle myrosinase belongs to the glycoside hydrolase family 1 and has up to 76% sequence similarity to other β-glucosidases. Phylogenetic analyses suggest species-specific diversification of this gene family in insects and an independent evolution of the beetle myrosinase from other insect β-glucosidases.

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 Dates: 20142014-05-20
 Publication Status: Published online
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 Identifiers: Other: BER001
DOI: 10.1073/pnas.1321781111
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 111 (20) Sequence Number: - Start / End Page: 7349 - 7354 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230