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  Reversible Membrane Pearling in Live Cells upon Destruction of the Actin Cortex

Heinrich, D., Ecke, M., Jasnin, M., Engel, U., & Gerisch, G. (2014). Reversible Membrane Pearling in Live Cells upon Destruction of the Actin Cortex. BIOPHYSICAL JOURNAL, 106(5), 1079-1091. doi:10.1016/j.bpj.2013.12.054.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-0CF9-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-1855-3
Genre: Journal Article

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 Creators:
Heinrich, Doris1, Author
Ecke, Mary2, Author              
Jasnin, Marion3, Author              
Engel, Ulrike1, Author
Gerisch, Günther2, Author              
Affiliations:
1external, ou_persistent22              
2Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              
3Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: DICTYOSTELIUM-DISCOIDEUM; PLASMA-MEMBRANE; PHASE-SEPARATION; LIVING CELLS; CYTOSKELETON DYNAMICS; CONTRACTILE VACUOLES; MOLECULAR MOTORS; BAR DOMAIN; CURVATURE; VESICLES
 Abstract: Membrane pearling in live cells is observed when the plasma membrane is depleted of its support, the cortical actin network. Upon efficient depolynnerization of actin, pearls of variable size are formed, which are connected by nanotubes of similar to 40 nm diameter. We show that formation of the membrane tubes and their transition into chains of pearls do not require external tension, and that they neither depend on microtubule-based molecular motors nor pressure generated by myosin-II. Pearling thus differs from blebbing. The pearling state is stable as long as actin is prevented from polymerizing. When polymerization is restored, the pearls are retracted into the cell, indicating continuity of the membrane. Our data suggest that the alternation of pearls and strings is an energetically favored state of the unsupported plasma membrane, and that one of the functions of the actin cortex is to prevent the membrane from spontaneously assuming this configuration.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000332501300011
DOI: 10.1016/j.bpj.2013.12.054
 Degree: -

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Title: BIOPHYSICAL JOURNAL
Source Genre: Journal
 Creator(s):
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 106 (5) Sequence Number: - Start / End Page: 1079 - 1091 Identifier: ISSN: 0006-3495