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  A Y2H-seq approach defines the human protein methyltransferase interactome

Weimann, M., Grossmann, A., Woodsmith, J., Özkan, Z., Birth, P., Meierhofer, D., et al. (2013). A Y2H-seq approach defines the human protein methyltransferase interactome. Nature methods, 10(4), 339-342. doi:10.1038/nmeth.2397.

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Weimann.pdf (Verlagsversion), 501KB
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© 2013 Macmillan Publishers Limited
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Weimann, M.1, Autor           
Grossmann, A.2, Autor           
Woodsmith, J.2, Autor           
Özkan, Z.1, Autor
Birth, P.1, Autor           
Meierhofer, D.3, Autor           
Benlasfer, N.2, Autor           
Valovka, T., Autor
Timmermann, B.4, Autor           
Wanker, E. E., Autor
Sauer, S.5, Autor           
Stelzl, U.2, Autor           
Affiliations:
1Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1433554              
2Molecular Interaction Networks (Ulrich Stelzl), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr, 73, 14195 Berlin, Germany, ou_1479660              
3Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              
4Sequencing (Head: Bernd Timmermann), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1479670              
5Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1479662              

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Schlagwörter: Chromatography, Liquid Escherichia coli Gene Expression Regulation, Enzymologic/physiology HEK293 Cells Humans Methyltransferases/genetics/*metabolism Protein Interaction Mapping/*methods Sensitivity and Specificity Sequence Analysis, DNA/methods Tandem Mass Spectrometry *Two-Hybrid System Techniques
 Zusammenfassung: To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.

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Sprache(n): eng - English
 Datum: 2013-03-032013-04
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1038/nmeth.2397
ISSN: 1548-7105 (Electronic)1548-7091 (Print)
URI: http://www.ncbi.nlm.nih.gov/pubmed/23455924
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Titel: Nature methods
  Andere : Nature methods
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: New York, NY : Nature Pub. Group
Seiten: - Band / Heft: 10 (4) Artikelnummer: - Start- / Endseite: 339 - 342 Identifikator: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556