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  A Y2H-seq approach defines the human protein methyltransferase interactome

Weimann, M., Grossmann, A., Woodsmith, J., Özkan, Z., Birth, P., Meierhofer, D., et al. (2013). A Y2H-seq approach defines the human protein methyltransferase interactome. Nature methods, 10(4), 339-342. doi:10.1038/nmeth.2397.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-0F4F-2 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-F04E-0
Genre: Journal Article

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 Creators:
Weimann, M.1, Author           
Grossmann, A.2, Author           
Woodsmith, J.2, Author           
Özkan, Z.1, Author
Birth, P.1, Author           
Meierhofer, D.3, Author           
Benlasfer, N.2, Author           
Valovka, T., Author
Timmermann, B.4, Author           
Wanker, E. E., Author
Sauer, S.5, Author           
Stelzl, U.2, Author           
Affiliations:
1Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1433554              
2Molecular Interaction Networks (Ulrich Stelzl), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr, 73, 14195 Berlin, Germany, ou_1479660              
3Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              
4Sequencing (Head: Bernd Timmermann), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1479670              
5Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1479662              

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Free keywords: Chromatography, Liquid Escherichia coli Gene Expression Regulation, Enzymologic/physiology HEK293 Cells Humans Methyltransferases/genetics/*metabolism Protein Interaction Mapping/*methods Sensitivity and Specificity Sequence Analysis, DNA/methods Tandem Mass Spectrometry *Two-Hybrid System Techniques
 Abstract: To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.

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Language(s): eng - English
 Dates: 2013-03-032013-04
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nmeth.2397
ISSN: 1548-7105 (Electronic)1548-7091 (Print)
URI: http://www.ncbi.nlm.nih.gov/pubmed/23455924
 Degree: -

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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 10 (4) Sequence Number: - Start / End Page: 339 - 342 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556