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  A lipid bound actin meshwork organizes liquid phase separation in model membranes.

Honigmann, A., Sadeghi, S., Keller, J., Hell, S. W., Eggeling, C., & Vink, R. (2014). A lipid bound actin meshwork organizes liquid phase separation in model membranes. eLife, 3: e01671. doi:10.7554/eLife.01671.001.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-1209-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-6DE5-9
Genre: Journal Article

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Honigmann, A.1, Author              
Sadeghi, S., Author
Keller, J.1, Author              
Hell, S. W.1, Author              
Eggeling, C.1, Author              
Vink , R., Author
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1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation. A minimal actin cortex was bound to a supported lipid bilayer via biotinylated lipid streptavidin complexes (pinning sites). In general, actin binding to ternary membranes prevented macroscopic liquid-ordered and liquid-disordered domain formation, even at low temperature. Instead, depending on the type of pinning lipid, an actin correlated multi-domain pattern was observed. FCS measurements revealed hindered diffusion of lipids in the presence of an actin network. To explain our experimental findings, a new simulation model is proposed, in which the membrane composition, the membrane curvature, and the actin pinning sites are all coupled. Our results reveal a mechanism how cells may prevent macroscopic demixing of their membrane components, while at the same time regulate the local membrane composition.

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Language(s): eng - English
 Dates: 2014-03-18
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.01671.001
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Title: eLife
Source Genre: Journal
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Pages: 16 Volume / Issue: 3 Sequence Number: e01671 Start / End Page: - Identifier: -