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  Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development

Pajoro, A., Madrigal, P., Muino, J. M., Matus, J. T., Jin, J., Mecchia, M. A., et al. (2014). Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development. Genome Biology, 15(3), R41-R41. doi:10.1186/gb-2014-15-3-r41.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-1B35-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-1B37-4
Genre: Journal Article

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 Creators:
Pajoro, A., Author
Madrigal, P., Author
Muino, J. M.1, Author              
Matus, J. T., Author
Jin, J., Author
Mecchia, M. A., Author
Debernardi, J. M., Author
Palatnik, J. F., Author
Balazadeh, S.2, Author              
Arif, M., Author
O'Maoileidigh, D. S., Author
Wellmer, F., Author
Krajewski, P., Author
Riechmann, J. L., Author
Angenent, G. C., Author
Kaufmann, K., Author
Affiliations:
1External Organizations, ou_persistent22              
2Transcription Factors and Gene Regulatory Networks, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753316              

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 Abstract: BACKGROUND: Development of eukaryotic organisms is controlled by transcription factors that trigger specific and global changes in gene expression programs. In plants, MADS-domain transcription factors act as master regulators of developmental switches and organ specification. However, the mechanisms by which these factors dynamically regulate the expression of their target genes at different developmental stages are still poorly understood. RESULTS: We characterized the relationship of chromatin accessibility, gene expression and DNA-binding of two MADS-domain proteins at different stages of Arabidopsis flower development. Dynamic changes in APETALA1 and SEPALLATA3 DNA-binding correlated with changes in gene expression, and many of the target genes could be associated with the developmental stage in which they are transcriptionally controlled. We also observe dynamic changes in chromatin accessibility during flower development. Remarkably, DNA-binding of APETALA1 and SEPALLATA3 is largely independent of the accessibility status of their binding regions and it can precede increases in DNA accessibility. These results suggest that APETALA1 and SEPALLATA3 may modulate chromatin accessibility, thereby facilitating access of other transcriptional regulators to their target genes. CONCLUSIONS: Our findings indicate that different homeotic factors regulate partly overlapping, yet also distinctive sets of target genes in a partly stage-specific fashion. By combining the information from DNA-binding and gene expression data, we are able to propose models of stage-specific regulatory interactions, thereby addressing dynamics of regulatory networks throughout flower development. Furthermore, MADS-domain TFs may regulate gene expression by alternative strategies, one of which is modulation of chromatin accessibility.

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Language(s): eng - English
 Dates: 2014-03-03
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1186/gb-2014-15-3-r41
ISSN: 1465-6914 (Electronic)1465-6906 (Print)
URI: http://www.ncbi.nlm.nih.gov/pubmed/24581456
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Title: Genome Biology
Source Genre: Journal
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Publ. Info: London : BioMed Central Ltd.
Pages: - Volume / Issue: 15 (3) Sequence Number: - Start / End Page: R41 - R41 Identifier: ISSN: 1465-6906
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000224390_1