English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Comprehensive dissection of spatio-temporal metabolic shifts in primary, secondary and lipid metabolism during developmental senescence in Arabidopsis thaliana

Watanabe, M., Balazadeh, S., Tohge, T., Erban, A., Giavalisco, P., Kopka, J., et al. (2013). Comprehensive dissection of spatio-temporal metabolic shifts in primary, secondary and lipid metabolism during developmental senescence in Arabidopsis thaliana. Plant Physiology, 162(3), 1290-1310. doi:10.1104/pp.113.217380.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-1BB6-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-1BB8-4
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Watanabe, M.1, Author              
Balazadeh, S.2, Author              
Tohge, T.3, Author              
Erban, A.4, Author              
Giavalisco, P.5, Author              
Kopka, J.4, Author              
Mueller-Roeber, B.6, Author              
Fernie, A. R.3, Author              
Hoefgen, R.1, Author              
Affiliations:
1Amino Acid and Sulfur Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753337              
2Transcription Factors and Gene Regulatory Networks, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753316              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
4Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              
5Experimental Systems Biology, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753342              
6Plant Signalling, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753311              

Content

show
hide
Free keywords: -
 Abstract: Developmental senescence is a coordinated physiological process in plants and is critical for nutrient redistribution from senescing leaves to newly formed sink organs including young leaves and developing seeds. Progress has been made concerning genes involved and the regulatory networks controlling senescence. The resulting complex metabolome changes during senescence have not been investigated in detail yet. Therefore, we conducted a comprehensive profiling of metabolites including pigments, lipids, sugars, amino acids, organic acids, nutrient ions, and secondary metabolites and determined approximately 260 metabolites at distinct stages in leaves and siliques during senescence in Arabidopsis thaliana. This provided an extensive catalogue of metabolites and their spatio-temporal co-behavior with progressing senescence. Comparison to silique data provides cues to source-sink relations. Further, we analysed the metabolite distribution within single leaves along the basipetal sink-source transition trajectory during senescence. Ceramids, lysolipids, aromatic amino acids, branched chain amino acids and stress induced amino acids accumulated, and an imbalance of Asn/Asp, Glu/Gln and nutrient ions in the tip region of leaves was detected. Furthermore, the spatio-temporal distribution of TCA cycle intermediates was already changed in the pre-senescent leaves and glucosinolates, raffinose and galactinol accumulated in the base region of leaves with proceeding senescence. These results are discussed in the context of current models of the metabolic shifts occurring during developmental and environmentally induced senescence. As senescence processes are correlated to crop yield, the metabolome data and the approach provided here can serve as a blueprint for the analysis of traits and conditions linking crop yield and senescence.

Details

show
hide
Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1104/pp.113.217380
BibTex Citekey: Watanabe21052013
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 162 (3) Sequence Number: - Start / End Page: 1290 - 1310 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438