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  Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment

Leinweber, M., Zmarz, P., Buchmann, P., Argast, P., Hübener, M., Bonhoeffer, T., et al. (2014). Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment. Journal of Visualized Experiments, 84: e50885. doi:10.3791/50885.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-7F6B-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-7FCF-5
Genre: Journal Article

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 Creators:
Leinweber, Marcus1, Author              
Zmarz, Pawel, Author
Buchmann, Peter, Author
Argast, Paul, Author
Hübener, Mark1, Author              
Bonhoeffer, Tobias1, Author              
Keller, Georg B.1, Author              
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1Department: Synapses-Circuits-Plasticity / Bonhoeffer, MPI of Neurobiology, Max Planck Society, ou_1113545              

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Free keywords: Behavior, Issue 84, Two-photon imaging, Virtual Reality, mouse behavior, adeno-associated virus, genetically encoded calcium indicators
 Abstract: In recent years, two-photon imaging has become an invaluable tool in neuroscience, as it allows for chronic measurement of the activity of genetically identified cells during behavior1-6. Here we describe methods to perform two-photon imaging in mouse cortex while the animal navigates a virtual reality environment. We focus on the aspects of the experimental procedures that are key to imaging in a behaving animal in a brightly lit virtual environment. The key problems that arise in this experimental setup that we here address are: minimizing brain motion related artifacts, minimizing light leak from the virtual reality projection system, and minimizing laser induced tissue damage. We also provide sample software to control the virtual reality environment and to do pupil tracking. With these procedures and resources it should be possible to convert a conventional two-photon microscope for use in behaving mice.

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Language(s): eng - English
 Dates: 2014-02-20
 Publication Status: Published online
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3791/50885
 Degree: -

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Title: Journal of Visualized Experiments
  Alternative Title : JoVE
Source Genre: Journal
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Pages: - Volume / Issue: 84 Sequence Number: e50885 Start / End Page: - Identifier: ISSN: 1940-087X