English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Differences in susceptibility and immune responses of three-spined sticklebacks (Gasterosteus aculeatus) from lake and river ecotypes to sequential infections with the eye fluke Diplostomum pseudospathaceum

Scharsack, J. P., & Kalbe, M. (2014). Differences in susceptibility and immune responses of three-spined sticklebacks (Gasterosteus aculeatus) from lake and river ecotypes to sequential infections with the eye fluke Diplostomum pseudospathaceum. Parasites & Vectors, 7: 109. doi:10.1186/1756-3305-7-109.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-88FC-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-88FF-0
Genre: Journal Article

Files

show Files
hide Files
:
Scharsack_2014.pdf (Publisher version), 2MB
Name:
Scharsack_2014.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Scharsack, Jörn Peter1, Author              
Kalbe, Martin2, Author              
Affiliations:
1Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445634              
2Research Group Parasitology, Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445643              

Content

show
hide
Free keywords: -
 Abstract: Abstract Background: The eye fluke Diplostomum pseudospathaceum is a frequent parasite of many fresh-water fish species, among those three-spined sticklebacks, particularly in lakes with lymnaeid snails, its first intermediate hosts. Cercariae released from host-snails, penetrate the skin of their fish hosts and within 24 h migrate to the immunologically inert eye lenses. Thus, individual D. pseudospathaceum are exposed to the fish immune system only for a short time, suggesting that only innate immunity can be active against the parasite. However, in nature sticklebacks are exposed to D. pseudospathaceum repeatedly since snails are shedding cercariae from late spring to autumn. Therefore, acquired immunity after initial infection would be advantageous against subsequent parasite encounters. Methods: We investigated if sticklebacks originating from a lake with high and from a river with low prevalence of D. pseudospathaceum differ in susceptibility to repeated exposure to the parasite. We compared infection success and immune functions in laboratory-bred sticklebacks from both habitats in naïve fish with fish that had been pre-exposed to eye flukes. Head kidney leukocytes (HKL) from experimental sticklebacks were investigated for respiratory burst activity and the proliferation of lymphocytes and monocytes 1.5, 5 and 15 days after infection. Results: Lake sticklebacks were less susceptible than river sticklebacks, however, in both populations pre-exposure led to a similar relative reduction in infection success. The respiratory burst activity was higher with HKL from lake sticklebacks and was up-regulated in pre-exposed fish but dropped 1.5d after an additional exposure, suggesting that activation of phagocytic cells is crucial for the defense against D. pseudospathaceum. Changes in lymphocyte proliferation were only detectable 1.5d after the last exposure in lake sticklebacks, but not 5 and 15d post exposure, indicating that a lymphocyte mediated acquired immune response was not induced. Proliferation of monocytes was significantly increased 1.5d after the last exposure with HKL from both stickleback populations. Conclusions: Increased resistance to D. pseudospathaceum in sticklebacks from both populations upon pre-exposure cannot be explained by a prominent adaptive immune response. Monocytic leukocytes were more responsive, suggesting that rather cells of the innate than the adaptive immune system are active in the defense of D. pseudospathaceum.

Details

show
hide
Language(s): eng - English
 Dates: 2013-11-012014-03-102014-03-21
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1186/1756-3305-7-109
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Parasites & Vectors
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : BioMed Central
Pages: 10 S. Volume / Issue: 7 Sequence Number: 109 Start / End Page: - Identifier: ISSN: 1756-3305
CoNE: /journals/resource/1756-3305