English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Tension and Force-Resistant Attachment Are Essential for Myofibrillogenesis in Drosophila Flight Muscle

Weitkunat, M., Kaya-Copur, A., Grill, S. W., & Schnorrer, F. (2014). Tension and Force-Resistant Attachment Are Essential for Myofibrillogenesis in Drosophila Flight Muscle. CURRENT BIOLOGY, 24(7), 705-716. doi:10.1016/j.cub.2014.02.032.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Weitkunat, Manuela1, Author              
Kaya-Copur, Aynur1, Author              
Grill, Stephan W.2, Author
Schnorrer, Frank1, Author              
Affiliations:
1Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565168              
2external, ou_persistent22              

Content

show
hide
Free keywords: PS INTEGRINS; CARDIAC MYOFIBRILLOGENESIS; EXTRACELLULAR-MATRIX; GENETIC-ANALYSIS; THICK FILAMENTS; DORSAL CLOSURE; IN-VIVO; M-LINE; SARCOMERE; MORPHOGENESIS
 Abstract: Background: Higher animals generate an elaborate muscle-tendon network to perform their movements. To build a functional network, developing muscles must establish stable connections with tendons and assemble their contractile apparatuses. Current myofibril assembly models do not consider the impact of muscle-tendon attachment on myofibrillogenesis. However, if attachment and myofibrillogenesis are not properly coordinated, premature muscle contractions can destroy an unstable myotendinous system, leading to severe myopathies. Results: Here, we use Drosophila indirect flight muscles to investigate how muscle-tendon attachment and myofibrillogenesis are coordinated. We find that flight muscles first stably attach to tendons and then assemble their myofibrils. Interestingly, this myofibril assembly is triggered simultaneously throughout the entire muscle, suggesting a self-assembly mechanism. By applying laser-cutting experiments, we show that muscle attachment coincides with an increase in mechanical tension before periodic myofibrils can be detected. We manipulated tension buildup within the myotendinous system either by genetically compromising attachment initiation and integrin recruitment to the myotendinous junction or by optically severing tendons from muscle. Both treatments cause strong myofibrillogenesis defects. We find that myosin motor activity is required for both tension formation and myofibril assembly, suggesting that myofibril assembly itself contributes to tension buildup. Conclusions: Our results demonstrate that force-resistant attachment enables a stark tension increase in the myotendinous system. Subsequently, this tension increase triggers simultaneous myofibril self-assembly throughout the entire muscle fiber. As myofibril and sarcomeric architecture as well as their molecular components are evolutionarily conserved, we propose a similar tension-based mechanism to regulate myofibrillogenesis in vertebrates.

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000333803200016
DOI: 10.1016/j.cub.2014.02.032
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: CURRENT BIOLOGY
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 24 (7) Sequence Number: - Start / End Page: 705 - 716 Identifier: ISSN: 0960-9822