English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  An optimized whole-body cortisol quantification method for assessing stress levels in larval zebrafish

Yeh, C.-M., Glöck, M., & Ryu, S. (2013). An optimized whole-body cortisol quantification method for assessing stress levels in larval zebrafish. PLoS One, 8(11), 1-8. doi:10.1371/journal.pone.0079406.

Item is

Files

show Files
hide Files
:
PLoSONE_8_2013_79406e.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
PLoSONE_8_2013_79406e.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Yeh, Chen-Min1, Author           
Glöck, Mario1, Author           
Ryu, Soojin1, Author           
Affiliations:
1Max Planck Research Group Developmental Genetics of the nervous system (Soojin Ryu), Max Planck Institute for Medical Research, Max Planck Society, ou_1497724              

Content

show
hide
Free keywords: -
 Abstract: Glucocorticoids serve important regulatory functions for many physiological processes and are critical mediators of the stress response. The stress response is a set of bodily processes aimed at counteracting a state of threatened homeostasis. Proper stress response is critical for the survival of an animal, however prolonged or abnormal stress response can be detrimental and is implicated in a number of human diseases such as depression and metabolic diseases. To dissect the underlying mechanism of this complex and important response, the zebrafish, Danio rerio offer important advantages such as ease of genetic manipulations and high-throughput behavioral analyses. However, there is a paucity of suitable methods to measure stress level in larval zebrafish. Therefore, an efficient low-cost method to monitor stress hormone levels will greatly facilitate stress research in zebrafish larvae. In this study, we optimized sample collection as well as cortisol extraction methods and developed a home-made ELISA protocol for measuring whole-body cortisol level in zebrafish larvae. Further, using our customized protocols, we characterized the response of larval zebrafish to a variety of stressors. This assay, developed for efficient cortisol quantification, will be useful for systematic and large-scale stress analyses in larval zebrafish.

Details

show
hide
Language(s): eng - English
 Dates: 2013-06-142013-09-302013-11-01
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1371/journal.pone.0079406
Other: 7935
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 8 (11) Sequence Number: - Start / End Page: 1 - 8 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850