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  A ternary AppA–PpsR–DNA complex mediates light regulation of photosynthesis-related gene expression

Winkler, A., Heintz, U., Lindner, R., Reinstein, J., Shoeman, R. L., & Schlichting, I. (2013). A ternary AppA–PpsR–DNA complex mediates light regulation of photosynthesis-related gene expression. Nature Structural and Molecular Biology, 20(7), 859-867. doi:10.1038/nsmb.2597.

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Alternative Title : A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression

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NatStructMolBiol_20_2013_859.pdf (Any fulltext), 3MB
 
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Winkler, Andreas1, Author              
Heintz, Udo1, Author              
Lindner, Robert1, Author              
Reinstein, Jochen1, Author              
Shoeman, Robert L.1, Author              
Schlichting, Ilme1, Author              
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources, depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the bacterium for using light energy. Illumination, however, enhances repression under semiaerobic conditions. Here, we describe molecular details of two proteins mediating oxygen and light control of photosynthesis-gene expression: the light-sensing antirepressor AppA and the transcriptional repressor PpsR. Our crystal structures of both proteins and their complex and hydrogen/deuterium-exchange data show that light activation of AppA–PpsR2 affects the PpsR effector region within the complex. DNA binding studies demonstrate the formation of a light-sensitive ternary AppA–PpsR–DNA complex. We discuss implications of these results for regulation by light and oxygen, highlighting new insights into blue light–mediated signal transduction

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Language(s): eng - English
 Dates: 2012-10-252013-04-102013-06-022013-07-01
 Publication Status: Published in print
 Pages: 9
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nsmb.2597
Other: 7907
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Title: Nature Structural and Molecular Biology
  Abbreviation : Nature Struct Biol
Source Genre: Journal
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Pages: - Volume / Issue: 20 (7) Sequence Number: - Start / End Page: 859 - 867 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763