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  BSH-CP based 3D solid-state NMR experiments for protein resonance assignment.

Shi, C., Fasshuber, H. K., Chevelkov, V., Xiang, S. Q., Habenstein, B., Vasa, S. K., et al. (2014). BSH-CP based 3D solid-state NMR experiments for protein resonance assignment. Journal of Biomolecular NMR, 59(1), 15-22. doi:10.1007/s10858-014-9820-8.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-908B-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-DD3F-F
Genre: Journal Article

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 Creators:
Shi, C.1, Author              
Fasshuber, H. K.1, Author              
Chevelkov, V.1, Author              
Xiang, S. Q.1, Author              
Habenstein, B.1, Author              
Vasa, S. K.1, Author              
Becker, S.2, Author              
Lange, A.1, Author              
Affiliations:
1Research Group of Solid-State NMR, MPI for biophysical chemistry, Max Planck Society, ou_persistent35              
2Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Free keywords: Solid-state NMR; Magic-angle spinning; BSH-CP; Protein resonance assignment; Ubiquitin
 Abstract: We have recently presented band-selective homonuclear cross-polarization (BSH-CP) as an efficient method for CO-CA transfer in deuterated as well as protonated solid proteins. Here we show how the BSH-CP CO-CA transfer block can be incorporated in a set of three-dimensional (3D) solid-state NMR (ssNMR) pulse schemes tailored for resonance assignment of proteins at high static magnetic fields and moderate magic-angle spinning rates. Due to the achieved excellent transfer efficiency of 33 % for BSH-CP, a complete set of 3D spectra needed for unambiguous resonance assignment could be rapidly recorded within 1 week for the model protein ubiquitin. Thus we expect that BSH-CP could replace the typically used CO-CA transfer schemes in well-established 3D ssNMR approaches for resonance assignment of solid biomolecules.

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Language(s): eng - English
 Dates: 2014-03-012014-05
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s10858-014-9820-8
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: 59 (1) Sequence Number: - Start / End Page: 15 - 22 Identifier: -