English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Gαq, Gγ1 and Plc21C control Drosophila body fat storage.

Baumbach, J., Xu, Y., Hehlert, P., & Kühnlein, R. P. (2014). Gαq, Gγ1 and Plc21C control Drosophila body fat storage. Journal of Genetics and Genomics, 41(5), 283-292. doi:10.1016/j.jgg.2014.03.005.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-9259-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CD85-1
Genre: Journal Article

Files

show Files
hide Files
:
2030612.pdf (Publisher version), 3MB
Name:
2030612.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2030612_Suppl_1.docx (Supplementary material), 112KB
Name:
2030612_Suppl_1.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2030612_Suppl_2.docx (Supplementary material), 20KB
Name:
2030612_Suppl_2.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2030612_Suppl_3.docx (Supplementary material), 24KB
Name:
2030612_Suppl_3.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Baumbach, J.1, Author              
Xu, Y.1, Author              
Hehlert, P.1, Author              
Kühnlein, R. P.1, Author              
Affiliations:
1Research Group of Molecular Physiology, MPI for biophysical chemistry, Max Planck Society, ou_578592              

Content

show
hide
Free keywords: Plc21C; Gαq; Gγ1; Calcium; Fat storage; Adipokinetic hormone
 Abstract: Adaptive mobilization of body fat is essential for energy homeostasis in animals. In insects, the adipokinetic hormone (Akh) systemically controls body fat mobilization. Biochemical evidence supports that Akh signals via a G protein-coupled receptor (GPCR) called Akh receptor (AkhR) using cyclic-AMP (cAMP) and Ca2+ second messengers to induce storage lipid release from fat body cells. Recently, we provided genetic evidence that the intracellular calcium (iCa2+) level in fat storage cells controls adiposity in the fruit fly Drosophila melanogaster. However, little is known about the genes, which mediate Akh signalling downstream of the AkhR to regulate changes in iCa2+. Here, we used thermogenetics to provide in vivo evidence that the GPCR signal transducers G protein α q subunit (Gαq), G protein γ1 (Gγ1) and Phospholipase C at 21C (Plc21C) control cellular and organismal fat storage in Drosophila. Transgenic modulation of Gαq, Gγ1 and Plc21C affected the iCa2+ of fat body cells and the expression profile of the lipid metabolism effector genes midway and brummer, which results in severely obese or lean flies. Moreover, functional impairment of Gαq, Gγ1 and Plc21C antagonised Akh-induced fat depletion. This study characterizes Gαq, Gγ1 and Plc21C as anti-obesity genes and supports the model that Akh employs the Gαq/Gγ1/Plc21C module of iCa2+ control to regulate lipid mobilization in adult Drosophila.

Details

show
hide
Language(s): eng - English
 Dates: 2014-03-272014-05-20
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.jgg.2014.03.005
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Genetics and Genomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 41 (5) Sequence Number: - Start / End Page: 283 - 292 Identifier: -