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  Tuning the electrical properties of the heart by differential trafficking of K-ATP ion channel complexes.

Arakel, E. C., Brandenburg, S., Uchida, K., Zhang, H., Lin, Y. W., Kohl, T., et al. (2014). Tuning the electrical properties of the heart by differential trafficking of K-ATP ion channel complexes. Journal of Cell Science, 127(9), 2106-2119. doi:10.1242/jcs.141440.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-B719-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-2285-1
Genre: Journal Article

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 Creators:
Arakel, E. C., Author
Brandenburg, S., Author
Uchida, K., Author
Zhang, H., Author
Lin, Y. W., Author
Kohl, T., Author
Schrul, B., Author
Sulkin, M. S., Author
Efimov, I., Author
Nichols, C. G., Author
Lehnart, S. E., Author
Schwappach, B.1, Author              
Affiliations:
1Max Planck Fellow Blanche Schwappach, ou_1548137              

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Free keywords: ATP-sensitive K+ channels; COPI; K-ATP; PKA; Trafficking; Protein kinase A; Cardiomyocyte; 14-3-3; Coatomer; Arg-based retrieval signal
 Abstract: The copy number of membrane proteins at the cell surface is tightly regulated. Many ion channels and receptors present retrieval motifs to COPI vesicle coats and are retained in the early secretory pathway. In some cases, the interaction with COPI is prevented by binding to 14-3-3 proteins. However, the functional significance of this antagonism between COPI and 14-3-3 in terminally differentiated cells is unknown. Here, we show that ATP-sensitive K+ (KATP) channels, which are composed of Kir6.2 and SUR1 subunits, are stalled in the Golgi complex of ventricular, but not atrial, cardiomyocytes. Upon sustained β-adrenergic stimulation, which leads to activation of protein kinase A (PKA), SUR1-containing channels reach the plasma membrane of ventricular cells. We show that PKA-dependent phosphorylation of the C-terminus of Kir6.2 decreases binding to COPI and, thereby, silences the arginine-based retrieval signal. Thus, activation of the sympathetic nervous system releases this population of KATP channels from storage in the Golgi and, hence, might facilitate the adaptive response to metabolic challenges.

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Language(s): eng - English
 Dates: 2014-02-252014-05-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1242/jcs.141440
 Degree: -

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Title: Journal of Cell Science
Source Genre: Journal
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Pages: - Volume / Issue: 127 (9) Sequence Number: - Start / End Page: 2106 - 2119 Identifier: -