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  Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.

Wilhelm, B. G., Mandad, S., Truckenbrodt, S., Kröhnert, K., Schäfer, C., Rammner, B., et al. (2014). Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science, 344(6187), 1023-1028. doi:10.1126/science.1252884.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-BF79-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CEAC-3
Genre: Journal Article

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 Creators:
Wilhelm, B. G., Author
Mandad, S.1, Author              
Truckenbrodt, S., Author
Kröhnert, K., Author
Schäfer, C., Author
Rammner, B., Author
Koo, S. J., Author
Classen, G. A., Author
Krauss, M., Author
Haucke, V., Author
Urlaub, H.1, Author              
Rizzoli, S. O., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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 Abstract: Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.

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Language(s): eng - English
 Dates: 2014-05-302014-05-30
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.1252884
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 344 (6187) Sequence Number: - Start / End Page: 1023 - 1028 Identifier: -