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  Subcellular localization, interactions and dynamics of the phage-shock protein-like Lia response in Bacillus subtilis

Dominguez-Escobar, J., Wolf, D., Fritz, G., Höfler, C., Wedlich-Söldner, R., & Mascher, T. (2014). Subcellular localization, interactions and dynamics of the phage-shock protein-like Lia response in Bacillus subtilis. MOLECULAR MICROBIOLOGY, 92(4), 716-732. doi:10.1111/mmi.12586.

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 Creators:
Dominguez-Escobar, Julia1, Author              
Wolf, Diana2, Author
Fritz, Georg2, Author
Höfler, Carolin2, Author
Wedlich-Söldner, Roland1, Author              
Mascher, Thorsten2, Author
Affiliations:
1Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              
2external, ou_persistent22              

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Free keywords: GREEN FLUORESCENT PROTEIN; SENSING 2-COMPONENT SYSTEM; DEPENDENT GENE-EXPRESSION; BACTERIAL 2-HYBRID SYSTEM; ENVELOPE STRESS-RESPONSE; GRAM-POSITIVE BACTERIA; CELL-WALL SYNTHESIS; ESCHERICHIA-COLI; YERSINIA-ENTEROCOLITICA; IN-VIVO
 Abstract: The liaIH operon of Bacillus subtilis is the main target of the envelope stress-inducible two-component system LiaRS. Here, we studied the localization, interaction and cellular dynamics of Lia proteins to gain insights into the physiological role of the Lia response. We demonstrate that LiaI serves as the membrane anchor for the phage-shock protein A homologue LiaH. Under non-inducing conditions, LiaI locates in highly motile membrane-associated foci, while LiaH is dispersed throughout the cytoplasm. Under stress conditions, both proteins are strongly induced and colocalize in numerous distinct static spots at the cytoplasmic membrane. This behaviour is independent of MreB and does also not correlate with the stalling of the cell wall biosynthesis machinery upon antibiotic inhibition. It can be induced by antibiotics that interfere with the membrane-anchored steps of cell wall biosynthesis, while compounds that inhibit the cytoplasmic or extracytoplasmic steps do not trigger this response. Taken together, our data are consistent with a model in which the Lia system scans the cytoplasmic membrane for envelope perturbations. Upon their detection, LiaS activates the cognate response regulator LiaR, which in turn strongly induces the liaIH operon. Simultaneously, LiaI recruits LiaH to the membrane, presumably to protect the envelope and counteract the antibiotic-induced damage.

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Language(s): eng - English
 Dates: 2014-05
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000335470300006
DOI: 10.1111/mmi.12586
 Degree: -

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Title: MOLECULAR MICROBIOLOGY
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 92 (4) Sequence Number: - Start / End Page: 716 - 732 Identifier: ISSN: 0950-382X