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  GroEL/ES Chaperonin Modulates the Mechanism and Accelerates the Rate of TIM-Barrel Domain Folding

Georgescauld, F., Popova, K., Gupta, A. J., Bracher, A., Engen, J. R., Hayer-Hartl, M., et al. (2014). GroEL/ES Chaperonin Modulates the Mechanism and Accelerates the Rate of TIM-Barrel Domain Folding. CELL, 157(4), 922-934. doi:10.1016/j.cell.2014.03.038.

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Georgescauld, Florian1, Autor           
Popova, Kristina1, Autor           
Gupta, Amit J.1, Autor           
Bracher, Andreas1, Autor           
Engen, John R.2, Autor
Hayer-Hartl, Manajit3, Autor           
Hartl, F. Ulrich1, Autor           
Affiliations:
1Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565152              
2external, ou_persistent22              
3Hayer-Hartl, Manajit / Chaperonin-assisted Protein Folding, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565153              

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Schlagwörter: MASS-SPECTROMETRY; HYDROGEN-EXCHANGE; ESCHERICHIA-COLI; IN-VIVO; E. COLI; PROTEIN; CONFINEMENT; RESOLUTION; KINETICS; MODEL
 Zusammenfassung: The GroEL/ES chaperonin system functions as a protein folding cage. Many obligate substrates of GroEL share the (beta alpha)(8) TIM-barrel fold, but how the chaperonin promotes folding of these proteins is not known. Here, we analyzed the folding of DapA at peptide resolution using hydrogen/deuterium exchange and mass spectrometry. During spontaneous folding, all elements of the DapA TIM barrel acquire structure simultaneously in a process associated with a long search time. In contrast, GroEL/ES accelerates folding more than 30-fold by catalyzing segmental structure formation in the TIM barrel. Segmental structure formation is also observed during the fast spontaneous folding of a structural homolog of DapA from a bacterium that lacks GroEL/ES. Thus, chaperonin independence correlates with folding properties otherwise enforced by protein confinement in the GroEL/ES cage. We suggest that folding catalysis by GroEL/ES is required by a set of proteins to reach native state at a biologically relevant time scale, avoiding aggregation or degradation.

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Sprache(n): eng - English
 Datum: 2014
 Publikationsstatus: Erschienen
 Seiten: 13
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000335765500019
DOI: 10.1016/j.cell.2014.03.038
 Art des Abschluß: -

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Titel: CELL
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Seiten: - Band / Heft: 157 (4) Artikelnummer: - Start- / Endseite: 922 - 934 Identifikator: ISSN: 0092-8674