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Free keywords:
HYPOXIC-NICHE; ACTIN; PROLIFERATION; MIGRATION; MOTILITY; BINDING;
MITOCHONDRIA; SURVIVAL; SUPPORTS; PROLINE
Abstract:
How stem cells interact with the microenvironment to regulate their cell fates and metabolismis largely unknown. Here we demonstrated that the deletion of the cytoskeleton modulating protein profilin 1 (pfn1) in hematopoietic stem cell (HSCs) led to bone marrow failure, loss of quiescence, and mobilization and apoptosis of HSCs in vivo. A switch from glycolysis to mitochondrial respiration with increased reactive oxygen species (ROS) level was also observed in HSCs on pfn1 deletion. Importantly, treatment of pfn1-deficient mice with the antioxidant N-acetyl-L-cysteine reversed the ROS level and loss of quiescence of HSCs, suggesting that the metabolism is mechanistically linked to the cell cycle quiescence of stem cells. The actin-binding and proline-binding activities of pfn1 are required for its function in HSCs. Our study provided evidence that pfn1 at least partially acts through the axis of pfn1/G alpha 13/EGR1 to regulate stem cell retention and metabolism in the bone marrow.
