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  Practical protocols for lipase immobilization via sol-gel techniques

Reetz, M. T. (2013). Practical protocols for lipase immobilization via sol-gel techniques. In J. M. Guisan (Ed.), Immobilization of Enzymes and Cells (pp. 241-254). Totowa: Humana >Press.

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 Creators:
Reetz, M. T.1, Author              
Affiliations:
1Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              

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Free keywords: Lipases; Sol-gel immobilization; Esterification; Transesterification; Thermal stability; Kinetic resolution; Enantioselectivity; Recyclability
 Abstract: Lipases can be efficiently entrapped in the pores of hydrophobic silicates by a simple and cheap sol–gel process in which a mixture of a hydrophobic alkylsilane RSi(OCH3)3 and Si(OCH3)4 is hydrolyzed under basic conditions in the presence of the enzyme. Additives such as isopropanol, polyvinyl alcohol, cyclodextrins, ionic liquids or surfactants enhance the efficiency of this type of lipase-immobilization. The main area of application of these heterogeneous biocatalysts concerns esterification or transesterification in organic solvents, ionic liquids, or supercritical carbon dioxide. Rate enhancements (relative to the traditional use of lipase powders) of several orders of magnitude have been observed, in addition to higher thermal stability. The lipase-immobilizates are particularly useful in the kinetic resolution of chiral esters, enantioselectivity often being higher than what is observed when using the commercial forms of these lipases (powder or classical immobilizates). Thus, due to the low price of sol–gel entrapment, the excellent performance of the lipase-immobilizates, and the ready recyclability, the method is industrially viable.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-62703-550-7_16
 Degree: -

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Title: Immobilization of Enzymes and Cells
Source Genre: Book
 Creator(s):
Guisan, J. M., Editor
Affiliations:
-
Publ. Info: Totowa : Humana >Press
Pages: 2. Edition Volume / Issue: - Sequence Number: - Start / End Page: 241 - 254 Identifier: -

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Title: Methods in Biotechnology
Source Genre: Series
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Publ. Info: -
Pages: - Volume / Issue: 1051 Sequence Number: - Start / End Page: - Identifier: -