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  From catalytic asymmetric synthesis to the transcriptional regulation of genes: In vivo and in vitro evolution of proteins

Barbas III, C. F., Rader, C., Segal, D. J., List, B., & Turner, J. M. (2001). From catalytic asymmetric synthesis to the transcriptional regulation of genes: In vivo and in vitro evolution of proteins. In F. H. Arnold (Ed.), Advances in Protein Chemistry, Vol. 55: Evolutionary Protein Design (pp. 317-366). New York: Academic Press.

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 Creators:
Barbas III, Carlos F:1, Author
Rader , Christopher1, Author
Segal, David J.1, Author
List, Benjamin1, Author              
Turner, James M.1, Author
Affiliations:
1The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA., ou_persistent22              

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 Abstract: This chapter focuses on the laboratory use of molecular evolution and discusses the selection to shape the novel proteins of defined function. The chapter discusses the types of function that involve the antibody/antigen interactions of the classical type (simple binding), the nonclassical type (catalysis), and the molecular recognition of long DNA addresses in complex genomes. The ability to manipulate the expression of specific endogenous genes would have wide-ranging applications in medicine and experimental and applied biology. In the therapy of cancer, for example, oncogenes, angiogenic factors, metastatic factors, and drug resistance genes are all potential targets for down regulation, while tumor suppressors, immunostimulants, and apoptotic factors could be targets for specific up regulation. Transcription factors are modular proteins consisting typically of a DNA-binding domain that provides for localization of the protein to a specific DNA address, and an effector domain that directs the type of activity to take place at the site. The wide range of effector activities available provide significant advantages over other proposed technologies.

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Language(s): eng - English
 Dates: 2001
 Publication Status: Published in print
 Pages: 50
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0065-3233(01)55008-1
 Degree: -

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Title: Advances in Protein Chemistry, Vol. 55: Evolutionary Protein Design
  Abbreviation : Adv. Protein Chem.
Source Genre: Series
 Creator(s):
Arnold, Frances H.1, Editor
Affiliations:
1 California Institute of Technology, Pasadena, California, USA, ou_persistent22            
Publ. Info: New York : Academic Press
Pages: - Volume / Issue: 55 Sequence Number: - Start / End Page: 317 - 366 Identifier: ISSN: 0065-3233
CoNE: https://pure.mpg.de/cone/journals/resource/954926956866