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  First steps towards underdominant genetic transformation of insect populations

Reeves, R. G., Bryk, J., Altrock, P. M., Denton, J. A., & Reed, F. A. (2014). First steps towards underdominant genetic transformation of insect populations. PLoS ONE, 9(5): e97557. doi:10.1371/journal.pone.0097557.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-D7D1-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-D7D2-6
Genre: Journal Article

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 Creators:
Reeves, R. Guy1, Author              
Bryk, Jarosłav2, Author              
Altrock, Philipp M.3, Author              
Denton, Jai A.2, Author              
Reed, Floyd A.1, Author              
Affiliations:
1Research Group Population Genetics, Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445646              
2Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
3Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445634              

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 Abstract: The idea of introducing genetic modifications into wild populations of insects to stop them from spreading diseases is more than 40 years old. Synthetic disease refractory genes have been successfully generated for mosquito vectors of dengue fever and human malaria. Equally important is the development of population transformation systems to drive and maintain disease refractory genes at high frequency in populations. We demonstrate an underdominant population transformation system in Drosophila melanogaster that has the property of being both spatially self-limiting and reversible to the original genetic state. Both population transformation and its reversal can be largely achieved within as few as 5 generations. The described genetic construct {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescue. In this proof-of-principle system the UAS-RpL14.dsRNA knock-down gene is placed under the control of an Actin5c-GAL4 driver located on a different chromosome to the {Ud} insert. This configuration would not be effective in wild populations without incorporating the Actin5c-GAL4 driver as part of the {Ud} construct (or replacing the UAS promoter with an appropriate direct promoter). It is however anticipated that the approach that underlies this underdominant system could potentially be applied to a number of species.

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Language(s): eng - English
 Dates: 2013-12-102014-04-082014-05-20
 Publication Status: Published online
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 Identifiers: DOI: 10.1371/journal.pone.0097557
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Title: PLoS ONE
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 9 (5) Sequence Number: e97557 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: /journals/resource/1000000000277850