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  Microfluidic cryofixation for correlative microscopy.

Mejia, Y. X., Feindt, H., Zhang, D., Steltenkamp, S., & Burg, T. P. (2014). Microfluidic cryofixation for correlative microscopy. Lab on a Chip, 14(17), 3281-3284. doi:10.1039/c4lc00333k.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-ED3F-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-5B8A-0
Genre: Journal Article

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 Creators:
Mejia, Y. X.1, Author              
Feindt, H., Author
Zhang, D., Author
Steltenkamp, S., Author
Burg, T. P.1, Author              
Affiliations:
1Research Group of Biological Micro- and Nanotechnology, MPI for biophysical chemistry, Max Planck Society, ou_578602              

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 Abstract: Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.

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Language(s): eng - English
 Dates: 2014-06-302014-09-07
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1039/c4lc00333k
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Title: Lab on a Chip
Source Genre: Journal
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Pages: - Volume / Issue: 14 (17) Sequence Number: - Start / End Page: 3281 - 3284 Identifier: -